Abstract
We report plant regeneration from maize (Zea mays L.) protoplasts with recovery of fertile plants from a Cateto inbred line adapted to tropical regions. Protoplasts were isolated from embryogenic cell suspension cultures capable of regenerating fertile plants. Cell colonies and embryogenic calli were obtained at high frequencies when protoplasts were cultured either in a thin layer of liquid medium or on cellulose-nitrate filters placed on a feeder layer of maize cells. High plating efficiencies were obtained with both culture methods using either a modified KM-8p or a modified N6 culture media. The results confirmed that maize protoplasts maintain the regeneration capacity of donor cells. We also demonstrated that high plating efficiencies and plant regeneration from maize protoplasts can be achieved with different culture methods. Furthermore, we recovered fertile plants with viable progenies from maize protoplasts.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Potrykus, I., Harms, C.T., Lörz, H. and Thomas, E. 1977. Callus formation from stem protoplasts of corn. Mol. Gen. Genet. 156:347–350.
Potrykus, I., Harms, C.T. and Lörz, H., 1979. Callus formation from cell culture protoplasts of corn (Zea mays L.). Theor. Appl. Genet. 54:209–214.
Chourey, P.S. and Zurawski, D.B. 1981. Callus formation from protoplasts of a maize cell culture. Theor. Appl. Genet. 59:341–344.
Ludwig, S.R., Somers, D.A., Petersen, W.L., Pohlman, R.F., Zarowits, M.A., Gengenbach, B.G., and Messing, J. 1985. High frequency callus formation for maize protoplasts. Theor. Appl. Genet. 71:344–350.
Imbrie-Milligan, C.W., Kamo, K.K., and Hodges, T.K. 1987. Microcallus growth from maize protoplasts. Planta 171:58–64.
Vasil, V. and Vasil, I.K. 1987. Formation of callus and somatic embryos from protoplasts of a commercial hybrid of maize (Zea mays L.). Theor. Appl. Genet. 73:793–798.
Kamo, K.K., Chang, K.L., Lynn, M.E., and Hodges, T.K. 1987. Embryogenic callus formation from maize protoplasts. Planta 172:245–251.
Rhodes, C.A., Lowe, K.S., and Ruby, K.L. 1988. Plant regeneration from protoplasts isolated from embryogenic maize cell cultures. Bio/Technology 6:56–60.
Shillito, R.D., Carswell, G.K., Johnson, C.M., DiMaio, J.J. and Harms, C.T. 1989. Regeneration of fertile plants from protoplasts of elite inbred maize. Bio/Technology 7, this issue.
Vasil, V. and Vasil, I.K. 1980. Isolation and culture of cereal protoplasts. II. Embryogenesis and plantlet formation from protoplasts of Penmsetum amencanum. Theor. Appl. Genet. 56:97–99.
Lu, C.Y., Vasil, V. and Vasil, I.K. 1981. Isolation and culture of protoplasts of Panicum maximum Jacq. (Guinea grass): somatic embryo-genesis and plantlet formation Z. Pflanzenphysiol. 104:311–318.
Vasil, V., Wang, D. and Vasil, I.K. 1983. Plant regeneration from protoplasts of Pennisetum purpureum Schum. (Napier grass). Z. Pflanzenphysiol. 111:319–325.
Fujimura, T., Sakurai, M., Agaki, H., Negishi, T. and Hirose, A. 1985. Regeneration of rice plants from protoplasts. Plant Tissue Culture Lett. 2:74–75.
Yamada, M., Yang, Z., and Tang, D. 1986. Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep. 5:85–88.
Kyozuka, J., Hayashi, Y. and Shimamoto, K. 1987. High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol. Gen. Genet. 206:408–413.
Srinivasan, C. and Vasil, I.K. 1986. Plant regeneration from protoplasts of sugarcane (Saccharum officinarum L.). J. Plant Physiol. 126:41–48.
Dalton, S.J. 1988. Plant regeneration from cell suspension protoplasts of Festuca arundinacea Schreb. (tall fescue) and Lohum perenne L. (perennial ryegrass). J. Plant Physiol. 132:170–175.
Horn, M.E., Conger, B.V., and Harms, C.T. 1988. Plant regeneration from protoplasts of embryogenic suspension cultures of orchard-grass (Dactylts glomerata L.). Plant Cell Rep. 7:371–374.
Beckert, M. and Qing, C.M. 1984. Results of a diallel trial and breeding experiment for in vitro aptitude in maize. Theor. Appl. Genet. 68:247–251.
Tomes, D.T. and Smith, O.S. 1985. The effect of parental genotype on initiation of embryogenic callus from elite maize (Zea mays L.) germplasm. Theor. Appl. Genet. 70:505–509.
Hodges, T.K., Kamo, K.K., Imbrie, C.W. and Becwar, M.R. 1986. Genotype specificity of somatic embryogenesis and regeneration in maize. Bio/Technology 4:219–223.
Prioli, L.M. and Silva, W.J. 1989. Somatic embryogenesis and plant regeneration capacity in tropical maize inbreds. Rev. Bras. Genet. In press.
Chu, C.C., Wang, C.C., Sun, C.S., Hsu, C., Yin, K.C., Chu, C.Y., and Bi, F.Y. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scientia Sinica 18:659–668.
Kao, K.N. and Michayluk, M.R. 1975. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at very low population density in liquid media. Planta 126:105–110.
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497.
Widholm, J.M. 1972. The use of fluorescein diacetate and phenosa-franine for determining viability of cultured plant cells. Stain Technology 47:189–194.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Prioli, L., Söndahl, M. Plant Regeneration and Recovery of Fertile Plants from Protoplasts of Maize (Zea Mays L.). Nat Biotechnol 7, 589–594 (1989). https://doi.org/10.1038/nbt0689-589
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/nbt0689-589
This article is cited by
-
Regeneration of cell suspension derived Apium graveolens L. protoplasts
Plant Cell, Tissue and Organ Culture (PCTOC) (2017)
-
Genetic engineering of cereal crop plants: a review
Euphytica (1995)