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Genetic Transformation of Banana and Plantain (Musa spp.) via Particle Bombardment

Bio/Technology volume 13, pages 481–485 (1995)Cite this article

Abstract

We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transient expression of the β-glucuronidase gene in both banana and plantain cells. Bombarded banana cells were selected with hygromycin and regenerated into plants. Molecular and histochemical characterization of transformants revealed the stable integration of the transferred genes into the banana genome.

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Authors and Affiliations

  1. Laboratory of Tropical Crop Husbandry, Catholic University of Leuven, K. Mercierlaan 92, B-3001, Leuven, Belgium

    László Sági, Bart Panis, Serge Remy, Hilde Schoofs, Kris De Smet & Rony Swennen

  2. F.A. Janssens Laboratory of Genetics, Catholic University of Leuven, W. de Croylaan 42, B-3001, Leuven, Belgium

    Bruno P.A. Cammue

Authors
  1. László Sági
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  2. Bart Panis
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  3. Serge Remy
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  4. Hilde Schoofs
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  6. Rony Swennen
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  7. Bruno P.A. Cammue
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Correspondence to László Sági.

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Sági, L., Panis, B., Remy, S. et al. Genetic Transformation of Banana and Plantain (Musa spp.) via Particle Bombardment. Nat Biotechnol 13, 481–485 (1995). https://doi.org/10.1038/nbt0595-481

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  • DOI: https://doi.org/10.1038/nbt0595-481

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