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Transgenic Tomato Plants Expressing the Tomato Yellow Leaf Curl Virus Capsid Protein are Resistant to the Virus

Bio/Technology volume 12, pages 500–504 (1994)Cite this article

Abstract

The tomato yellow leaf curl virus(TYLCV) gene that encodes the capsid protein (VI) was placed under transcriptional control of the cauliflower mosaic virus 35S promoter and cloned into an Agrobacterium Ti-derived plasmid and used to transform plants from an interspecific tomato hybrid, Lycopersicon esculentum X L. pennellii (F1), sensitive to the TYLCV disease. When transgenic F1 plants, expressing the V1 gene, were inoculated with TYLCV using whiteflies fed on TYLCV-infected plants, they responded either as untransformed tomato or showed expression of delayed disease symptoms and recovery from the disease with increasingly more resistance upon repeated inoculation. Transformed plants that were as sensitive to inoculation as untransformed controls expressed the V1 gene at the RNA level only. All the transformed plants that recovered from disease expressed the TYLCV capsid protein.

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Author notes

  1. Yedidya Gafni: e-mail: VCGAFNI@VOLCANI.

Authors and Affiliations

  1. Department of Genetics, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel

    Talya Kunik & Yedidya Gafni

  2. Department of Virology, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel

    Raffi Salomon

  3. Department of Field and Vegetable Crops and the Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Faculty of Agriculture, P.O. Box 12, Rehovot, 76100, Israel

    Daniel Zamir, Nir Navot, Muhammad Zeidan, Ilana Michelson & Henryk Czosnek

Authors
  1. Talya Kunik
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  2. Raffi Salomon
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  3. Daniel Zamir
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  4. Nir Navot
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  5. Muhammad Zeidan
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  6. Ilana Michelson
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  7. Yedidya Gafni
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  8. Henryk Czosnek
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Kunik, T., Salomon, R., Zamir, D. et al. Transgenic Tomato Plants Expressing the Tomato Yellow Leaf Curl Virus Capsid Protein are Resistant to the Virus. Nat Biotechnol 12, 500–504 (1994). https://doi.org/10.1038/nbt0594-500

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  • DOI: https://doi.org/10.1038/nbt0594-500

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