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Efficient Production of Antibody Fragments by the Filamentous Fungus Trichoderma reesei

Bio/Technology volume 11, pages 591–595 (1993)Cite this article

Abstract

We have engineered the filamentous fungus Trichoderma reesei to assemble and secrete immunologically authentic engineered Fab antibody fragments into the culture medium. A major improvement in yield was achieved by fusing the heavy Fd chain to the T. reesei cellulase, CBHI. The yields of secreted, immunologically active Fab and CBHI-Fab fusion were 1 mg/1 and 150 mg/1, respectively. The Fab fragment can be released from the fusion protein CBHI-Fab by an extracellular T. reesei protease. There was no detectable difference in affinity for the antigen between the engineered Fab and the idiotypic antibody.

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Author information

Author notes

  1. Eini Nyyssönen

    Present address: Genencor International Inc., 180 Kimball Way, South San Francisco, CA, 94080

  2. Anu Harkki

    Present address: Cultor Ltd., Technology Center, Kantvik, SF-02460, Finland

  3. Sirkka Keränen: Corresponding author.

Authors and Affiliations

  1. VTT Biotechnical Laboratory, P.O. Box 202, SF-02151, Espoo, Finland

    Eini Nyyssönen, Merja Penttilä, Anu Harkki, Anu Saloheimo, Jonathan K. C. Knowles & Sirkka Keränen

  2. Glaxo Institute for Molecular Biology, 14 chemin des Aulx, 1228, Plan-les-Ouates. Geneva, Switzerland

    Jonathan K. C. Knowles

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  1. Eini Nyyssönen
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Nyyssönen, E., Penttilä, M., Harkki, A. et al. Efficient Production of Antibody Fragments by the Filamentous Fungus Trichoderma reesei. Nat Biotechnol 11, 591–595 (1993). https://doi.org/10.1038/nbt0593-591

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