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Stable Yeast Transformants that Secrete Functional α–Amylase Encoded by Cloned Mouse Pancreatic cDNA

Bio/Technology volume 4, pages 311–315 (1986)Cite this article

Abstract

Mouse pancreatic α–amylase complementary DNA was inserted into a yeast shuttle vector after the Saccharomyces cerevisiae MFα1 promoter and secretion signals coding sequences. When transformed with the recombinant plasmid, S. cerevisiae cells were able to synthesize and secrete functional α–amylase, efficiently hydrolysing starch present in the culture medium. Stable amylolytic cells were obtained from different yeast strains. This work represents a significant step towards producing yeast that can convert starchy materials directly to ethanol.

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Author notes

  1. Elisabete V. Galembeck, Juarez B. Faria and Ana C. Schenberg Frascino: Universidade de São Paulo, Instituto de Química, Caixa Postal 20780, 01498 São Paulo, SP, Brasil

Authors and Affiliations

  1. Instituto de Ciências Biológicas, Departamento de Biologia Celular; 70910 Brasília, Universidade de Brasília, DF, Brazil

    Spartaco Astolfi Filho

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  1. Spartaco Astolfi Filho
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  2. Elisabete V. Galembeck
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  3. Juarez B. Faria
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  4. Ana C. Schenberg Frascino
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Filho, S., Galembeck, E., Faria, J. et al. Stable Yeast Transformants that Secrete Functional α–Amylase Encoded by Cloned Mouse Pancreatic cDNA. Nat Biotechnol 4, 311–315 (1986). https://doi.org/10.1038/nbt0486-311

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