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Transgenic Indica Rice Breeding Line IR58 Expressing a Synthetic crylA(b) Gene from Bacillus thuringiensis Provides Effective Insect Pest Control

Bio/Technology volume 14, pages 171–176 (1996)Cite this article

Abstract

The Indica rice breeding line IR58 was transformed by particle bombardment with a truncated version of a synthetic crylA(b) gene from Bacillus thuringiensis. This gene is expressed under control of the CaMV 35S promoter and allows efficient production of the lepidopteran specific δ-endotoxin. Ro, Rx and R2 generation plants displayed a significant insecticidal effect on several lepidopterous insect pests. Feeding studies showed mortality rates of up to 100% for two of the most destructive insect pests of rice in Asia, the yellow stem borer (Scirpophaga incertulas?) and the striped stem borer (Chilo suppressalis), and feeding inhibition of the two leaffolder species Cnaphalocrocis medinalis and Marasmia patnalis. Introduction of stem borer resistance into the germplasm of an Indica rice breeding line now makes this agronomically important trait available for conventional rice breeding programs.

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Author information

Author notes

  1. Gadab C. Ghosh Biswas

    Present address: The Norman Borlaug Institute for Plant Science Research, De Montfort University, Scraptoft, Leicester, LE7 9SU, England

  2. Victor A. Iglesias

    Present address: Institute of Molecular Biology, Austrian Academy of Sciences, A-8020, Salzburg

  3. Joachim Wünn: Corresponding author: ETH Zurich, Institute of Plant Sciences, CH-8092 Zurich, Switzerland.

Authors and Affiliations

  1. ETH Zurich, Institute of Plant Sciences, CH-8092, Zurich, Switzerland

    Andreas Klöti, Peter K. Burkhardt & Ingo Potrykus

  2. CIBA-GEIGY, Research Triangle Park, NC, 27709, USA

    Karen Launis

Authors
  1. Joachim Wünn
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  7. Ingo Potrykus
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Wünn, J., Klöti, A., Burkhardt, P. et al. Transgenic Indica Rice Breeding Line IR58 Expressing a Synthetic crylA(b) Gene from Bacillus thuringiensis Provides Effective Insect Pest Control. Nat Biotechnol 14, 171–176 (1996). https://doi.org/10.1038/nbt0296-171

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