Abstract
Among the biological products whose production involves animal cell cultivation techniques are viral vaccines (human and veterinary), monoclonal antibodies, cell surface antigens, enzymes, polypeptide growth factors, hormones, immunoregulators, viral bioinsecticides, tumor antigens, cell mass as a product and reconstitution of living skin. The R&D and production of these substances is a complicated art requiring knowledge and experience in one or more of the following advanced technologies: 1. Hybridization techniques. 2. Development of novel as well as modification of conventional cultivation equipment. 3. Development of a wide range of microcarriers. 4. Adaptation of microencapsulation technology. 5. Intensive development of serum-free and/or low cost media for cultivation of animal cells. 6. Advances in protein concentration and purification techniques.
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References
Margaritis, A. and Wallace, J.B. 1984. Novel bioreactor systems and their applications. Bio/Technology 2:447–453.
Reuveny, S. 1983. Microcarriers for culturing mammalian cells and their applications, p. 1–32. In: Advances in Biotechnological Processes, Vol. 2, Mizrahi, A. and Van Wezel, A. L. (eds.). Alan Liss. New York, N.Y.
Reuveny, S. 1985. Microcarriers in cell culture: Structure and applications. Adv. Cell Culture 4:213–247.
Schleicher, J.B. and Weiss, R.E. 1968. Application of a multi-surface tissue culture propagator for the production of cell monolayers, virus, and biochemicals. Biotec. Bioeng. 10:617–624.
House, W., Shearer, M. and Maroudas, N.G. 1972. Method for bulk culture of animal cells on plastic film. Exp. Cell Res. 71:293–296.
Nardelli, L. and Panina, G.F. 1976. 10-years experience with a 28,000 roller bottle plant for FMD vaccine production. Develop. Biol. Stand. 37:133–138.
Knakek, R.A., Gullino, P.M., Kohler, P.O., and Dedrick, R.L. 1972. Cell culture on artificial capillaries: An approach to tissue growth in vitro. Science 175:65–67.
Whiteside, J.P., Withing, B.R., and Spier, R.E. 1979. Development of a methodology for the production of Foot-and-Mouth Disease virus from BHK 21 C13 monolayer cell growth in a 100 1 (20m2) glass spheric propagator. Develop. Biol. Stand. 42:113–120.
Jensen, M.D. 1977. Mass Cell Culture in a Controlled Environment, p. 589–602. In: Cell Culture and its Application, Acton, R. T. and Lynn, J. D. (eds.) Academic Press, New York, NY.
Feder, J. and Tolbert, W.R. 1983. The large-scale cultivation of mammalian cells. Sci. Am. 248:24–31.
Feder, J.B. and Tolbert, W.R. 1985. Mass culture of mammalian cells in perfusion systems. Intern. Biotechnol. Lab. 3:40–53.
Weimann, M.C., Creswick, B., and Calabresi, P. 1984. Cellular and biochemical aspects of human tumor cell growth and function in hollow fiber culture. 188th. ACS National Meeting Philadelphia, PA. Abstract ♯15.
Boraston, R., Thompson, P.W., Garland, S., and Birch, J.R. 1982. Growth and oxygen requirements of antibody producing mouse hybridoma cells in suspension culture. Develop. Biol. Standard. 55:103–111.
Scheirer, W., Nilsson, K., Merten, O.W., Katinger, H.W.D., and Mosbach, K. 1982. Entrapment of animal cells for the production of biomolecules such as monoclonal antibodies. Develop. Biol. Stand. 55:155–161.
Lim, F. and Moss, R.D. 1981. Microencapsulation of living cells and tissues. J. Pharma. Sci. 70:351–354.
Buckland, B.C. 1984. The translation of scale in fermentation processes: The impact of computer process control. Bio/Technology 2:875–882.
Jayme, D.W. and Blackman, K.E. 1985. Review of culture media for propagation of mammalian cells, viruses and other biologicals, p. 2–31. In: Advances in Biotechnological Processes, Vol. 5, Mizrahi, A. and Van Wezel, A. L. (eds.) Alan Liss, New York, N.Y.
Chang, T.H., Steplewski, Z. and Koyzowski, H. 1980. Production of monoclonal antibodies in serum free medium. J. Immunol. Methods 39:369–375.
Murakami, H., Masui, H. and Sato, G.H. 1982. Suspension culture of hybridoma cells in serum-free medium: Soybean phaspholipids as the essential components. Cold Spring Harbor Conference on Cell Proliferation 9:711–715.
Darfler, F.J. and Insel, P.A. 1982. Growth of normal and transformed lymphoid cells and hybridomas in serum-free medium. Cold Spring Harbor Conferences on Cell Proliferation 9:717–726.
Rodriguez, H., Enriquez, A. and Volfova, O. 1983. Optimization of culture medium composition for cellulolytic bacteria by mathematical methods. Folia Microbiol. 28:163–171.
Diamond, W.J. 1981. Practical experiment designs for engineers and scientists, Lifetime Learning Publications, Belmont, CA, U.S.A.
Zimmerman, U., Vienken, J., Halfmann, J., and Emeis, C.C. 1985. Electrofusion: A novel hybridization technique, p. 79–150. In: Advances in Biotechnological Processes, Vol. 4, Mizrahi, A. and Van Wezel, A. L. (eds.) Alan Liss, New York, N.Y.
Ben Nathan, D. 1985. Production of poultry viral vaccines in embryonated eggs, p. 124–148. In: Advances in Biotechnological Processes, Vol. 5, Mizrahi, A. and Van Wezel, A. L. (eds.) Alan Liss, New York, N.Y.
McAleer, W.J., Buynak, E.B., Weibel, R.E., Villarejos, V.M., Scattergood, E.M., Wasmith, E.H., McLean, A.A., and Hilleman, M.R. 1975. Measles, mumps and rubella virus vaccines prepared from virus prepared by unit process. J. Biol. Standard. 3:381–384.
Spier, R.E. 1983. Production of Veterinary vaccines, p. 33–59. In: Advances in Biotechnological Processes, Vol. 2, Mizrahi, A. and Van Wezel, A. L. (eds.). Alan Liss, New York, N.Y.
Dumonde, D.C., Wolstencroft, R.A., Panayi, G.S., Matthew, M., Morley, J., and Howson, W.T. 1969. “Lymphokines”: Non-antibody mediators of cellular immunity generated by lymphocyte activation. Nature 224:38–42.
Rocklin, R.E., Bendtzen, K., and Greineder, D. 1981. Mediators of immunity: Lymphokines and monokines, p. 55–136. In: Advances in Immunology, Vol. 29, Kunkel, H. G. and Dixon, F. J. (eds.). Academic Press. New York, N.Y.
Leong, S.S. and Horoszewitz, J.S. 1981. Production and preparation of human fibroblast interferon, p. 87–101. In: Methods in Enzymology. Vol. 78 (Part A), Pestka, S. (ed.). Academic Press. New York, N.Y.
Cantel, K. and Hirvonen, S. 1978. Large-scale production of human leukocyte containing 108 units per ml. J. Gen. Virol. 39:541–543.
Fanilletti, P.C. 1983. Production and concentration of interferon from human leukocytes, p. 169–177. In: Advances in Biotechnological Processes, Vol. 2, Mizrahi, A. and Van Wezel, A. L. (eds.). Alan R. Liss, New York, N.Y.
Lazar, A. 1983. Interferon production by human lymphoblastoid cells, p. 179–208. In: Advances in Biotechnological Processes, Vol. 2, Mizrahi, A. and Van Wezel, A. L. (eds.) Alan R. Liss, New York, N.Y.
Mizrahi, A., Reuveny, S., Traub, A., Minai, M. 1980. Large-scale production of human lymphoblastoid (Namalva) interferon I. Production of crude interferon. Biotechnol. Lett. 2:267–271.
Klein, R., Ricketts, R.T., Jones, W.I., De Armon, I.A., Temple, M.J., Zoon, K.C., and Bridgen, P.J. 1979. Large scale production and concentration of human lymphoid interferon. Antimicrob. Agents Chemother. 15:420–427.
Shoham, J. 1983. Production of human immune interferon, p. 209–269. In: Advances in Biotechnological Processes, Vol. 2, Mizrahi, A. and Van Wezel, A. L. (eds.) Alan Liss, New York, N.Y.
Wanger, H. and Rollinglaff, M. 1978. T-T cell interactions during in-vitro cytotoxic allograft responses. J. Exp. Med. 148:1523–1528.
Fresno, M., Nabel, G., McVay-Boudreau, L., Furthmayer, H., and Cantor, H. 1981. Antigen specific T-lymphocyte clones. J. Exp. Med. 153:1246–1259.
Klein, F., Ricketts, R., Pickle, D., and Flickinger, M.C. 1983. Interleukin-2 and interleukin-3: Suspension cultures of constitutive producer cell lines, p. 111–134. In: Advances in Biotechnological Processes, Vol. 2, Mizrahi, A. and Van Wezel, A. L. (eds.). Alan Liss, New York, N.Y.
Gillis, S. and Watson, J. 1980. Biochemical and biological characterization of lymphocyte regulatory molecules. J. Exp. Med. 152:1709–1719.
Pawelec, G., Borowitz, A., Krammer, H., and Wernet, P. 1982. Constitutive interleukin-2 production by the JURKAT human leukemic T-cell line. Europ. J. Immunol. 12:380–386.
Stanley, E.R., Cifone, M., Heard, P.M., and Defendi, V. 1976. Factors regulating macrophage production and growth: Identity of colony-stimulating factor and macrophage growth factor. J. Exp. Med. 143:637–647.
Okabe, T., Nomura, H., Sato, N., Ohsawa, N. 1982. Large-scale preparation and characterization of human colony-stimulating factor. J. Cell Physiol. 110:43–49.
Okabe, T. and Ohsawa, N. 1981. Human colony-stimulating factor producing cell line. Cell. Molecular Biol. 27:579–587.
Soredni, B., Sieckmann, D.G., Kumagais, S., House, S., Green, I., and Paul, W.E. 1981. Long-term culture and cloning of non-transformed human B lymphocytes, J. Exp. Med. 154:1500–1516.
Takatsu, K., Harada, N., Hara, Y., Takaham, Y., Yamada, G., Dobashi, K., and Hamaoka, T. 1985. Purification and physicochemical characterization of murine T-cell replacing factor (TRF). J. Immunol. 134:382–389.
Reuveny, D., Velez, D., Riske, R., McMillan, J.D., and Miller, L. 1985. Production of monoclonal antibodies in culture. Develop. Biol. Stand. In press.
Gardina, T.A., and Jarvis, A.P. 1984. Microencapsulation of human X human cells. Comparison of cell growth and monoclonal antibody production between microcapsule and conventional suspension cultures. Hybridoma 3:73.
Strand, J.M., Quarles, J.M., and McConnell, S. 1982. Matrix Perfusion-Microcarrier Bead Culture of Anchorage Dependent Cells. In Vitro 18:311.
Seaver, S.S. and Gabriels, J.E. 1985. Monoclonal Antibody Production: Stirred culture vs. Novel membrane perfusion. ASM Annual Meeting. Abstract ♯22.
Boraston, R., Thompson, P.W., Garland, S., and Birch, J.R. 1982. Growth and oxygen requirements of antibody producing mouse hybridoma cells in suspension culture. Develop. Biol. Stand. 55:103–111.
Carpenter, G. and Cohen, S. 1979. Epidermal growth factor. Ann. Rev. Biochem. 48:193–216.
Rheimwald, J.G. and Green, H. 1977. Epidermal growth factor and multiplication of cultured human epidermal keratinocytes. Nature 265:421–424.
Stiles, C.D. 1983. The molecular biology of platelet-derived growth factor. Cell 33:653–655.
Rinderknecht, E. and Humbel, R.E. 1978. The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin. J. Biol. Chem. 253:2769–2776.
Rinderknecht, E. and Humbel, R.E. 1978. Primary structure of human insulin-like growth factor II. FEBS Lett. 89:283–286.
Marquardt, H., Hunkapiller, M.W., Hood, L.E., Twarozik, D.R., DeLance, J.E., Stephenson, J.R., and Todaro, G.J. 1983. Transforming growth factor produced by retrovirus transformed rodent fibroblasts and human melanoma cells: Amino acid sequence homology with epidermal growth factor. Proc. Natl. Acad. Sci. 80:4684–4688.
Angenetti, R.H. and Bradshaw, R.A. 1971. Nerve growth factor from mouse submaxillary gland: Amino acid sequence. Proc. Natl. Acad. Sci. 68:2417–2420.
Thomas, K.A., Baylan, N.C., and Bradshaw, R.A. 1981. The amino acid sequence of the j-subunit of mouse submaxillary gland 7S nerve growth factor. J. Bio. Chem. 256:9156–9166.
Hunter, T. 1984. The proteins of oncogenes. Sci. Am. 251:60–69.
Kris, R.M., Libermann, T.A., Avivi, A., and Schlesinger, J. 1985. Growth Factors, growth-factor receptors and oncogenes. Bio/Technology 3:135–140.
Feramisce, J., Ozanne, B., and Stiles, C.D. 1985. Cancer Cell 3/Growth Factors and Transformation. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Green, H., Kehinde, O., and Thomas, J. 1979. Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc. Natl. Acad. Sci. 76:5665–5669.
Katinger, H.W.D. and Blien, R. 1983. Production of enzymes and hormones by mammalian cell culture, p. 61–95. Advances in Biotechnological Processes, Vol. 2, Mizrahi, A. and Van Wezel, A. L. (eds.). Alan Liss, New York, N.Y.
Kaduri, A. and Bohak, Z. 1985. Production of plasminogen activator by cells in culture. p. 275–292. In: Advances in Biotechnological Processes, Vol. 5, Mizrahi, A. and Van Wezel, A. L. (eds.). Alan Liss, New York, N.Y.
Kluft, C., Van Wezel, A.L., Van Der Velden, C.A.M., Encis, J.J., Verheijen, J.H., and Wijngaards 1983. Large-scale production of extrinsic (tissue-type) plasminogen activators from human melanoma cells, p. 97–110. In: Advances in Biotechnological Processes, Vol. 2, Mizrahi, A. and Van Wezel, A. L. (eds.). Alan Liss, New York, N.Y.
Miltenburger, H.G. and Krieg, A. 1984. Bioinsecticides II. Baculoviridae, p. 291–313. In: Advances in Biotechnological Process, Vol. 3, Mizrahi, A. and Van Wezel, A. L. (eds.). Alan Liss, New York, N.Y.
Lazar, A., Reuveny, S., Geva, Y., Ganor, Y., Marcus, D., Ariel, N., Epstein, N., Altboum, Z., Sinai, J., and Mizrahi, A. 1985. Production of carcinoembryonic antigen by a human colon carcinoma cell line grown on microcarriers. J. Natl. Cancer Inst. Submitted.
Kam, W., Tsao, D., Itzkowitz, S.H., and Kim, Y.S. 1985. Production, Characterization and clinical utility of carcinoembryonic antigen, p. 151–181. In: Advances in Biotechnological Processes, Vol. 4, Mizrahi, A. and Van Wezel, A. L. (eds.). Alan Liss, New York, N.Y.
Bell, E., Sher, S., Hull, B., Merrill, C., Rosen, S., Chanson, A., Asselinean, D., Dubertret, L., Ceulomb, B.C., Lapiere, C., Nusgens, B., and Nevenx, Y. 1983. The reconstitution of living skin. J. Investigative Dermatol. 81:2S–10S.
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Mizrahi, A. Biologicals from Animal Cells in Culture. Nat Biotechnol 4, 123–127 (1986). https://doi.org/10.1038/nbt0286-123
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DOI: https://doi.org/10.1038/nbt0286-123