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Knockout mice created by TALEN-mediated gene targeting

Nature Biotechnology volume 31, pages 23–24 (2013)Cite this article

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Phenotypic analysis of gene-specific knockout mice has transformed our understanding of in vivo gene functions. Generation of knockout mice, however, remains a time-consuming and expensive process. Transcription activator-like (TAL) effector nucleases (TALENs) are highly effective in inducing mutations at specific genomic loci1,2, and consequently TALEN-mediated mutagenesis in zygotes is a potential alternative to conventional gene targeting in mice. However, to the best of our knowledge, gene knockout mice have yet to be created using TALENs. Here, we report the generation of mice with a genetic knockout of the progesterone immunomodulatory binding factor 1(Pibf1) or selenoprotein W, muscle 1 (Sepw1) gene using TALENs.

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Figure 1: Generation of TALEN-mediated Pibf1 mutant mice.

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Acknowledgements

H.-W.L. was supported by the MEST (20120006489, 20120000174, 2012009607) and by the MHW (A085136), Republic of Korea. J.-S.K. was supported by the NRF (2012-0001225). Y.H.S. and D.H.K. are supported by the BK21 program.

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Author notes

  1. Young Hoon Sung, In-Jeoung Baek and Duk Hyoung Kim: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea

    Young Hoon Sung, Jisun Jeon, Jaehoon Lee & Han-Woong Lee

  2. Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Republic of Korea

    In-Jeoung Baek

  3. Department of Chemistry, Seoul National University, Seoul, Republic of Korea

    Duk Hyoung Kim & Jin-Soo Kim

  4. Department of Microbiology and Aging-Associated Vascular Disease Research Center, Yeungnam University, Daegu, Republic of Korea

    Kyunghee Lee & Daewon Jeong

  5. Laboratory Animal Research Center, Yonsei University, Seoul, Republic of Korea

    Han-Woong Lee

Authors
  1. Young Hoon Sung
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  2. In-Jeoung Baek
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  4. Jisun Jeon
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  8. Jin-Soo Kim
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Correspondence to Jin-Soo Kim or Han-Woong Lee.

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Sung, Y., Baek, IJ., Kim, D. et al. Knockout mice created by TALEN-mediated gene targeting. Nat Biotechnol 31, 23–24 (2013). https://doi.org/10.1038/nbt.2477

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