Abstract
The ability of organs such as the liver or the lymphoid system to maintain their original size or regain it after injury is well documented1,2. However, little is known about how these organs sense that equilibrium is breached, and how they cease changing when homeostasis is reached. Similarly, it remains unclear how, during normal development, different cell types within an organ coordinate their growth. Here we show that during gonad development in the fruitfly Drosophila melanogaster the proliferation of primordial germ cells (PGCs) and survival of the somatic intermingled cells (ICs) that contact them are coordinated by means of a feedback mechanism composed of a positive signal and a negative signal. PGCs express the EGF receptor (EGFR) ligand Spitz, which is required for IC survival. In turn, ICs inhibit PGC proliferation. Thus, homeostasis and coordination of growth between soma and germ line in the larval ovary is achieved by using a sensor of PGC numbers (EGFR-mediated survival of ICs) coupled to a correction mechanism inhibiting PGC proliferation. This feedback loop ensures that sufficient numbers of PGCs exist to fill all the stem-cell niches that form at the end of larval development. We propose that similar feedback mechanisms might be generally used for coordinated growth, regeneration and homeostasis.
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Acknowledgements
We thank J. Treisman for her support with both materials and ideas; G. Dietzl and B. Dickson for sharing UAS-SpiRNAi before publication; B. Edgar and L. Johnston for materials; J. Lafaille for discussions; S. Burden and J. Morris for critical reading of the manuscript; and C. Navarro, D. Siekhaus and all members of the Lehmann laboratory for comments on the manuscript. The Bloomington Stock Center provided reagents. L.G. is supported by a fellowship from the Helen and Martin Kimmel Center for Stem Cell Biology. R.L. is a Howard Hughes Medical Institute investigator.
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Supplementary Figure 1
This Supplementary Figure shows the gonadal expression pattern of the protein Traffic Jam during larval development in wild type and in EgfrCA gonads. (PDF 96 kb)
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Gilboa, L., Lehmann, R. Soma–germline interactions coordinate homeostasis and growth in the Drosophila gonad. Nature 443, 97–100 (2006). https://doi.org/10.1038/nature05068
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DOI: https://doi.org/10.1038/nature05068
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