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Restricted growth of Schwann cells lacking Cajal bands slows conduction in myelinated nerves


Nerve impulses are propagated at nodes of Ranvier in the myelinated nerves of vertebrates. Internodal distances have been proposed to affect the velocity of nerve impulse conduction1; however, direct evidence is lacking, and the cellular mechanisms that might regulate the length of the myelinated segments are unknown. Ramón y Cajal described longitudinal and transverse bands of cytoplasm or trabeculae in internodal Schwann cells and suggested that they had a nutritive function2. Here we show that internodal growth in wild-type nerves is precisely matched to nerve extension, but disruption of the cytoplasmic bands in Periaxin-null mice impairs Schwann cell elongation during nerve growth. By contrast, myelination proceeds normally. The capacity of wild-type and mutant Schwann cells to elongate is cell-autonomous, indicating that passive stretching can account for the lengthening of the internode during limb growth. As predicted on theoretical grounds, decreased internodal distances strikingly decrease conduction velocities and so affect motor function. We propose that microtubule-based transport in the longitudinal bands of Cajal permits internodal Schwann cells to lengthen in response to axonal growth, thus ensuring rapid nerve impulse transmission.

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Figure 1: Longitudinal and transverse bands of Cajal in Schwann cells and their disruption in quadriceps nerve of Periaxin-null (KO) mice at 3 weeks.
Figure 2: Internodal length is decreased in Schwann cells from Prx-/- mice lacking Cajal bands.
Figure 3: The capacity of Schwann cells to specify internodal length is cell-autonomous.
Figure 4: Intact Cajal bands and microtubules are required for MBP mRNA localization at the paranodes.
Figure 5: Peripheral nerve function is compromised in Prx-/- animals.


  1. Brill, M. H., Waxman, S. G., Moore, J. W. & Joyner, R. W. Conduction velocity and spike configuration in myelinated fibres: computed dependence on internode distance. J. Neurol. Neurosurg. Psychiatry 40, 769–774 (1977)

    CAS  Article  Google Scholar 

  2. Ramón y Cajal, S. Histology (Bailliere, Tindall & Cox, London, 1933)

    Google Scholar 

  3. Sherman, D. L., Fabrizi, C., Gillespie, C. S. & Brophy, P. J. Specific disruption of a Schwann cell dystrophin-related protein complex in a demyelinating neuropathy. Neuron 30, 677–687 (2001)

    CAS  Article  Google Scholar 

  4. Gillespie, C. S. et al. Peripheral demyelination and neuropathic pain behavior in periaxin-deficient mice. Neuron 26, 523–531 (2000)

    CAS  Article  Google Scholar 

  5. Pratt, T., Sharp, L., Nichols, J., Price, D. J. & Mason, J. O. Embryonic stem cells and transgenic mice ubiquitously expressing a tau-tagged green fluorescent protein. Dev. Biol. 228, 19–28 (2000)

    CAS  Article  Google Scholar 

  6. Ainger, K. et al. Transport and localization elements in myelin basic protein mRNA. J. Cell Biol. 138, 1077–1087 (1997)

    CAS  Article  Google Scholar 

  7. Carson, J. H., Worboys, K., Ainger, K. & Barbarese, E. Translocation of myelin basic protein mRNA in oligodendrocytes requires microtubules and kinesin. Cell Motil. Cytoskeleton 38, 318–328 (1997)

    CAS  Article  Google Scholar 

  8. Colman, D. R., Kreibich, G., Frey, A. B. & Sabatini, D. D. Synthesis and incorporation of myelin polypeptides into CNS myelin. J. Cell Biol. 95, 598–608 (1982)

    CAS  Article  Google Scholar 

  9. Griffiths, I. R. et al. Expression of myelin protein genes in Schwann cells. J. Neurocytol. 18, 345–352 (1989)

    CAS  Article  Google Scholar 

  10. Anzini, P. et al. Structural abnormalities and deficient maintenance of peripheral nerve myelin in mice lacking the gap junction protein connexin 32. J. Neurosci. 17, 4545–4551 (1997)

    CAS  Article  Google Scholar 

  11. Hursh, J. B. Conduction velocity and diameter of nerve fibers. Am. J. Physiol. 127, 131–139 (1939)

    Article  Google Scholar 

  12. Huxley, A. F. & Stampfli, R. Evidence for saltatory conduction in peripheral myelinated nerve fibres. J. Physiol. (Lond.) 108, 315–339 (1949)

    Article  Google Scholar 

  13. McIntyre, C. C., Richardson, A. G. & Grill, W. M. Modeling the excitability of mammalian nerve fibers: influence of afterpotentials on the recovery cycle. J. Neurophysiol. 87, 995–1006 (2002)

    Article  Google Scholar 

  14. Ranvier, L. Des étranglements annulaires et des segments interannulaires chez les Raies et les Torpilles. C. R. Acad. Sci. 75, 1129–1132 (1872)

    Google Scholar 

  15. Hiscoe, H. B. Distribution of nodes and incisures in normal and regenerated nerve. Anat. Rec. 99, 447–475 (1947)

    CAS  Article  Google Scholar 

  16. Schlaepfer, W. W. & Myers, F. K. Relationship of myelin internode elongation and growth in the rat sural nerve. J. Comp. Neurol. 147, 255–266 (1973)

    CAS  Article  Google Scholar 

  17. Tait, S. et al. An oligodendrocyte cell adhesion molecule at the site of assembly of the paranodal axo-glial junction. J. Cell Biol. 150, 657–666 (2000)

    CAS  Article  Google Scholar 

  18. Komada, M. & Soriano, P. βIV-spectrin regulates sodium channel clustering through ankyrin-G at axon initial segments and nodes of Ranvier. J. Cell Biol. 156, 337–348 (2002)

    CAS  Article  Google Scholar 

  19. Gillespie, C. S., Sherman, D. L., Blair, G. E. & Brophy, P. J. Periaxin, a novel protein of myelinating Schwann cells with a possible role in axonal ensheathment. Neuron 12, 497–508 (1994)

    CAS  Article  Google Scholar 

  20. Trapp, B. D. et al. Polarization of myelinating Schwann cell surface membranes: role of microtubules and the trans-Golgi network. J. Neurosci. 15, 1797–1807 (1995)

    CAS  Article  Google Scholar 

  21. Collinson, J. M., Marshall, D., Gillespie, C. S. & Brophy, P. J. Transient expression of neurofascin by oligodendrocytes at the onset of myelinogenesis: implications for mechanisms of axon-glial interaction. Glia 23, 11–23 (1998)

    CAS  Article  Google Scholar 

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We thank H. Anderson and L. Ferguson for assistance, B. Smith for technical support, S. Scherer for comments, and K. Willeke and T. Ott of Bonn University for the CMTX mice. Figure 1a is reproduced with the permission of the Cajal Institute, CSIC, Madrid, Spain, copyright inheritors of Santiago Ramón y Cajal. This work was supported by the Wellcome Trust.

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Correspondence to Peter J. Brophy.

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Court, F., Sherman, D., Pratt, T. et al. Restricted growth of Schwann cells lacking Cajal bands slows conduction in myelinated nerves. Nature 431, 191–195 (2004).

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