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Diagnosis | Rhabdomyosarcoma

Lab Animal volume 39, pages 138–139 (2010)Cite this article

Immunohistochemistry, a method of identifying cell types based on the binding of antibodies to specific cell-type components, has emerged as a valuable adjunct to hematoxylin and eosin staining in diagnostic histopathology. We used avidin–biotin–peroxidase complex (Vectastain, ABC Kit Elite; Vector Corporation, Burlingame, CA, USA) immunohistochemistry on serial sections of the tumor. The antibodies we used were mouse monoclonal antibody to human keratin (clone AE1/AE3, Dako, Denmark) diluted 1:100, mouse monoclonal antibody to human vimentin (VIM 3B4 code M7020, Dako, Denmark) diluted 1:100, mouse monoclonal antibody to human actin (clone HHF35, Enzo Life Sciences, USA) diluted 1:50 and rabbit polyclonal S100 protein (Z0311, Dako, Denmark) diluted 1:200.

Macroscopic findings in the present case are in general accordance with those previously described of rhabdomyosarcoma in mice1,2. The tumor presented as a raised, firm mass in an area where skeletal muscle is normally located. Most cases are described with intact overlying skin, whereas this mouse had ulcerated skin overlying the tumor. When sectioned, the tumor was solid and speckled, dark red in appearance. In one report3, 14 well-differentiated rhabdomyosarcomas were diagnosed in 10,000 necropsies of mice. The most frequent location (10 of 14) was the quadriceps muscles.

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Figure 2: Microscopic appearance of the mass in Figure 1, showing bundles of fusiform cells with eosinophilic cytoplasm and blunt-ended nuclei alternating with round cells.
Figure 3: Immunohistochemical staining of the spindle cell tumor in Figure 2, showing expression of actin in the cytoplasm of the neoplastic fusiform cells.

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Acknowledgements

This work has been supported by grant number P07-CVI-02559 from the Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain.

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Diagnosis | Rhabdomyosarcoma. Lab Anim 39, 138–139 (2010). https://doi.org/10.1038/laban0510-138

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