Abstract
We previously reported that the blood–brain barrier (BBB) function was deteriorated in vessels located in the hippocampus, but not the cerebral cortex, in 3-month-old stroke-prone spontaneously hypertensive rats (SHRSP). Recently published data suggest that matrix metalloproteinase (MMP)-2 and MMP-9 play a critical role in the BBB disruption in stroke or cerebral ischemia. In this study, we examined gene and protein expressions of MMPs in the BBB-damaged hippocampal vessels of 3-month-old SHRSP, in the cerebral cortical vessels without BBB damage of SHRSP, and in the hippocampal and cerebral cortical ones without BBB damage of 3-month-old Wistar Kyoto (WKY) rats. The expressions of MMPs were examined by real-time quantitative reverse transcriptase-PCR (RT-PCR), western blotting and immunohistochemical techniques. The gene and protein expressions of MMP-13 were significantly increased in the hippocampal samples of SHRSP compared with samples without BBB damage, such as cerebral cortical samples of SHRSP or hippocampal samples of WKY. Immunostaining of MMP-13 was seen in the cytoplasm of ED-1-positive perivascular cells in both rats and was colocalized with those of type IV collagen or osteopontin. The type IV collagen was also localized in the basement membrane. These findings indicate that the expression of MMP-13 is increased in BBB-damaged hippocampal vessels in hypertensive SHRSP compared with vessels without BBB impairment in normotensive WKY rats and may be involved in vascular remodeling.
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Introduction
A causative role of blood–brain barrier (BBB) impairment is suggested in the pathogenesis of vascular dementia with leakage of serum components from small vessels leading to neuronal and glial damage.1 To clarify the roles of BBB impairment in vascular dementia, we have examined BBB function in various conditions related to vascular dementia, such as hypertension, acute ischemia with reperfusion, chronic cerebral hypoperfusion and aging with or without memory deficits.2, 3, 4 Consequently, among these animal models, the most localized BBB impairment was clearly seen in the hippocampus, but not the cerebral cortex, of 3-month-old spontaneously hypertensive rats (SHR), especially of 3-month-old stroke-prone SHR (SHRSP),4 which were established by Okamoto and Aoki5 and are described in detail elsewhere.6, 7 It is known that the neuronal loss occurs with a reduction of gray matter volume in the CA1 subfield and dentate gyrus of the hippocampus in SHR at the age of 6 months or order, indicating that SHR can be a good animal model of vascular dementia.8, 9 SHRSP is also well known as an excellent model for subcortical vascular dementia accompanied by small-vessel pathology, because these rats show structural alterations of small cerebral arteries,10 reduction in cerebral blood flow,11 white matter lesions12, 13 and cognitive impairment.14, 15 We then examined differences in gene expression between microdissected hippocampal vessels with BBB impairment in SHRSP and those without the impairment in Wistar Kyoto (WKY) rats using a microarray assay and reported that the expression of osteopontin, a matricellular protein, was increased in BBB-damaged vessels in hypertensive SHRSP compared with that in vessels without BBB impairment in WKY rats.16 The study also indicated that the immunoreactivity of osteopontin was present in perivascular ED1-positive macrophage/microglial cells and suggested the possibility that osteopontin might act as an inducible modulator of vascular remodeling, which likely restored BBB function in the damaged blood vessels. In addition, we recently reported17 that the expression of P-glycoprotein, a representative efflux transporter in the brain vessels, was increased in vessels with BBB impairment in the hippocampus of SHRSP. However, it remains to be clarified how damaged BBB function is restored or modulated in the hippocampal vessels of SHRSP.
Matrix metalloproteinases (MMPs) are proteolytic enzymes and involved in the remodeling of the extracellular matrix in a variety of physiological and pathophysiological conditions. Among MMPs, MMP-1 is known as type I collagenase, whereas gelatinase A (MMP-2) and gelatinase B (MMP-9), type IV collagenases, are able to digest the endothelial basal lamina leading to the opening of the BBB.18, 19, 20 Expression of MMP-9 and MMP-2 has been shown to be significantly increased during stroke in human21 and animal models of focal ischemia.19, 20, 22, 23 MMP-9 is of particular interest in the context of acute brain ischemia, because selective upregulation of MMP-9 has been observed in the brains of patients with stroke.24 Activation of MMP-9 with increased BBB permeability contributes to the early formation of vasogenic edema after focal cerebral ischemia.25, 26 Direct intracerebral injection of one of the MMPs, MMP-2, caused an increase in capillary permeability and hemorrhage.18 In addition, it is known that MMP-2 plays a critical role in the BBB disruption, glial activation and white matter lesions after chronic cerebral hypoperfusion.27, 28 In the rat model of chronic cerebral hypoperfusion, BBB function was disrupted in the white matter lesions.3 These results suggest that increased expression of MMP-2 and MMP-9 induce increased BBB permeability followed by brain damage. In addition, it was reported using an MMP protein array that high levels of MMP-9 and MMP-13, also known as collagenase-3, are involved in diffusion-weighted image lesion growth.29 It remains to be clarified whether MMP-13 is involved in the BBB disruption.
In this study, we examined whether the expression of some MMPs increased in BBB-damaged vessels of hypertensive animal models.
Methods
Experimental animals and procedures
The experimental protocols for animal care were in accordance with the National Research Council’s Guidelines. Three-month-old male SHRSP/Izm (n=14) and WKY/Izm (n=14) rats (purchased from Japan SLC Inc., Hamamatsu, Japan) weighing 250–380 g were used. All efforts were made to minimize the number of animals used and their suffering. Systolic and diastolic arterial pressure was measured three times in each conscious rat by tail-cuff plethysmography (BP-98A; Softron Co., Tokyo, Japan) and the mean values were calculated. The animals were anesthetized with diethyl ether and then used for several experiments.
For real-time quantitative reverse transcriptase-PCR (RT-PCR) and western blot analyses, rats were perfused transcardially with 0.01 mol l−1 phosphate-buffered saline (PBS). Their brains were removed, quickly cut into several regions including the frontal cortex and the hippocampus and stored at −80°C. For immunocytochemical examination, rats were perfused transcardially with PBS and then with a fixative containing 4% paraformaldehyde in 0.1 mol l−1 phosphate buffer (pH 7.4). The brains were removed and immersed in the same fixative.
Real-time quantitative RT-PCR
Total RNA was extracted from the hippocampus and the frontal cortex with Trizol, according to the manufacturer’s instructions. The RNA concentrations were determined by spectroscopy at 260 nm. Reverse transcription of total RNA (5 μg) was performed with a hexa-deoxyribonucleotide mixture (0.35 μg) as primer, and Moloney murine leukemia virus-reverse transcriptase and ribonuclease inhibitor (human placenta) at 37°C for 60 min. The reaction was terminated by cooling at 70°C for 15 min. To quantify gene expression, TaqMan real-time quantitative PCR was performed with the ABI PRISM 7700 Sequence Detection System using Assays on-Demand Gene Expression probes of MMPs (MMP-2, MMP-3, MMP-9 and MMP-13), or glyceraldehydes 3-phosphate dehydrogenase (GAPDH) and TaqMan Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA), as reported earlier.30 The PCR cycling conditions for all samples were as follows: 50°C, 2 min for AmpErase UNG activation; 95°C, 10 min for AmpliTaq Gold activation; and 50 cycles for melting (95°C, 15 s) and annealing/extension (60°C, 1 min) steps.16, 31 Each sample was run in duplicate, and each PCR experiment included two nontemplate control wells. The comparative threshold cycle method (Applied Biosystems) was used to calculate gene expression in each sample relative to the value observed in control samples using GAPDH as a control for normalization among samples.
Western blot analysis
Western blot analysis was performed to evaluate the expression of various proteins. The brain tissues were homogenized in lysis buffer containing 10 mmol l−1 Tris buffer containing 2 mmol l−1 phenylmethylsulfonyl fluoride, and 10 mmol l−1 N-ethylmaleimide with a protease inhibitor. The protein concentration was determined with the Bradford reagent spectrophotometrically at A595. The extracts were solubilized in a Tris/glycine/sodium dodecyl sulfate sample buffer in the presence of 5% 2-mercaptoethanol (Bio-Rad, Hercules, CA, USA) and heated at 95°C for 5 min. Protein extracts (40 μg) were then separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Next, the separated proteins were transferred from gel to nitrocellulose membranes and blocked in a blocking solution (5% dry milk in PBS) for 1 h. Subsequently, each membrane was incubated with a primary antibody at 4°C overnight. The primary antibodies used in western blotting analysis were rabbit anti-MMP-1 (1:1000, Biomol, Plymouth Meeting, PA, USA), rabbit anti-MMP-2 (1:200, Santa Cruz, Santa Cruz, CA, USA), rabbit anti-MMP9 (1:200, Santa Cruz) and mouse anti-MMP-13 (1:200, Chemicon International, Temecula, CA, USA) antibodies. The membranes were then incubated with horseradish peroxidase-labeled anti-rabbit IgG or anti-mouse IgG antibody (1:5000 or 1:2000, Amersham Biosciences, Buckinghamshire, UK) for 1 h at room temperature. The proteins were visualized on enhanced chemiluminescence film (Hyperfilm; Amersham Biosciences) by applying them to a Western Blotting Detection System (Amersham Biosciences). Finally, the blots were reprobed using an antibody against α-tubulin (1:100, Santa Cruz), followed by incubation in biotin-conjugated anti-mouse IgM antibody (1:500, Chemicon International) and then in horseradish peroxidase-conjugated anti-biotin antibody (1:1000, Cell Signaling, Danvers, MA, USA). Data are expressed as the relative differences among samples after normalization to α-tubulin expression. The immunoreactive bands were analyzed with a LAS1000plus lumino-imaging analyzer (Fuji Film, Tokyo, Japan).
Immunohistochemistry
For triple staining, the tissue sections were treated with 10% normal serum and incubated with the mouse anti-MMP-13 antibody (1:200, Chemicon) or a goat anti-MMP-13 antibody (1:200, Chemicon) at 4°C overnight, followed by incubation in Alexa Fluor 488-conjugated anti-mouse IgG (1:200, Molecular Probes, Eugene, OR, USA) or in Rhodamin-conjugated anti-goat IgG antibody (1:200, Chemicon). After washing in PBS, the sections were incubated with rabbit anti-type IV collagen (1:100, Santa Cruz), rabbit anti-osteopontin antibody (1:200, Rockland, Gilbertsville, PA, USA) or mouse anti-ED1 antibody (1:200, Serotec Ltd, Oxford, UK) at 4°C overnight, followed by incubation in Alexa Fluor 594-conjugated anti-rabbit IgG (1:200, Molecular Probes) or in fluorescein isothiocyanate-conjugated anti-mouse IgG antibody (1:200, Vector laboratories, Burlingame, CA, USA). Cross-reactions were not detected after double staining. In addition, the sections were incubated for 20 min in 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes) solution at room temperature, which was diluted to 400 nM in 1% albumin solution in PBS. The fluorescent signals were viewed under a confocal microscope (Bio-Rad Radiance 2100). As a control experiment, we performed an identical immunohistochemical procedure with omission of the primary antibody or using a normal serum instead of the primary antibody.
Statistical analysis
The values are shown as means±s.d. Statistical analysis was performed with two-way analysis of variance followed by the Tukey method. Differences were considered to be statistically significant when P<0.05.
Results
Blood pressure
Blood pressure was significantly higher in 3-month-old SHRSP (210±14/174±12 mm Hg) than WKY rats (131±10/101±10 mmHg) of the same age.
Real-time quantitative RT-PCR analysis
Real-time quantitative RT-PCR was performed to compare the quantity of gene expression of MMP-2, MMP-3, MMP-9 and MMP-13 among the hippocampal and cerebral cortical samples of SHRSP and WKY rats. MMP-2 gene expression was increased in hippocampal samples of SHRSP (1.5-fold) or WKY rats (2.2-fold) compared with cerebral cortical samples in rats of corresponding strains, although there were no significant differences in gene expression between rats of both strains (Figure 1a). There were no significant differences in MMP-3 gene expression among the samples in four groups (Figure 1b). MMP-9 gene expression was decreased in hippocampal samples of SHRSP (0.58-fold) or WKY (0.73-fold) compared with cerebral cortical samples in rats of corresponding strains, although there were no significant differences in gene expression between rats of both strains (Figure 1c). MMP-13 gene expression was increased in hippocampal samples of SHRSP compared with hippocampal samples of WKY (3.2-fold) or with cerebral cortical samples of SHRSP rats (2.5-fold) (Figure 1d).
Western blot analysis
Western blot analysis was performed to compare the quantity of protein expression of MMP-1, MMP-2, MMP-9 and MMP-13 among the hippocampal and cerebral cortical samples of SHRSP and WKY rats. There were no significant differences in MMP-1, MMP-2 and MMP-9 protein expression among samples in four groups (Figures 2a–c). Western blot analysis in MMP-13 expression showed that a major and a minor band were located at molecular weights of approximately 45–50 and 20–25 kDa (Figure 2d), respectively. The density in the minor band was increased in the hippocampal samples of SHRSP compared with the hippocampal ones of WKY rats (1.4-fold) (Figure 2d), although there were no significant differences in the density of the major band among samples in four groups (data not shown).
Immunohistochemical analysis
The immunostaining of MMP-13 was colocalized with that in the cytoplasm of ED1-positive perivascular cells in rats of both strains, indicating that MMP-13 is present in the cytoplasm of perivascular cells of microglia/microphage lineage (Figures 3a and b). The immunostaining of MMP-13 was also colocalized with that of collagen (Figures 3c and d) or osteopontin (Figures 3e and f). The type IV collagen was also localized in the basement membrane. There was no clear regional difference in the MMP-13 immunopositive structures between SHRSP and WKY rats (Figure 3).
Discussion
The earlier study4 showed that the BBB function was deteriorated in vessels located along hippocampal fissures of 3-month-old SHRSP, but not in same-age WKY rats. The present study indicated that the gene and protein expression of MMP-13 were increased in hippocampal samples in SHRSP compared with those in WKY rats and that MMP-13 was present in the cytoplasm of perivascular cells of microglia/macrophage lineage. In this study, the immunostaining for MMP-13 was not clear in the endothelial cells of vessels. But, the role of MMP-13 in the endothelial cells cannot be disregarded because MMP-13 in endothelial cells is known to play a role in protection of atherosclerosis,30 which may affect barrier function. Nagel et al.32 noted that two processed forms (47 and 20 kDa) of MMP-13 appeared in the brain after ischemia. These findings suggest that the MMP-13 expression is increased in the BBB-damage hippocampal vessels of SHRSP. Increased expression of MMP-13, also known as collagenase-3, may degrade the components of basement membranes of the hippocampal vessels, presumably followed by contribution to BBB damage.
We earlier reported4 that the BBB function was deteriorated in vessels located in the hippocampus, but not the cerebral cortex, in 3-month-old SHRSP. The reason for the regional difference is not clear. The hippocampus is supplied by the middle cerebral artery, especially proximal portions of it. It is thought that the middle cerebral arteries of SHR are proximal resistance arteries, exposed to major increases in blood pressure, and show increased resting tone and reduced arterial distensibility.33, 34 Accordingly, it is likely that these factors affect BBB function in the hippocampus of hypertensive rats. Effects of genetic factors cannot be disregarded.
The earlier study4 also showed that collagen deposits were occasionally seen in the BBB-damaged vessels. In addition, Iwanaga et al.16 microdissected vascular samples from the hippocampus of SHRSP and WKY rats and examined the difference in gene expression between the vascular samples with BBB impairment of SHRSP and those without impairment of WKY, using a microarray assay. Accordingly, osteopontin expression was increased in BBB-damaged hippocampal vessels in SHRSP, compared with hippocampal vessels without the BBB impairment in WKY rats.8 In this study, the expression of MMP-13 was colocalized with those of osteopontin as well as collagen, suggesting that MMP-13 expression is increased in BBB-damaged vessels with vascular collagenosis or remodeling. In addition, the results in the microarray assay also indicated that the expression of transforming growth factor-β receptor II was increased in the hippocampal samples of SHRSP, compared with that in the samples of WKY rats.16 It is known that transforming growth factor-β enhances MMP-13 expression.35, 36 The increased expression of MMP-13 together with transforming growth factor-β receptor II in the BBB-damaged vessels of SHRSP is compatible with these findings reported earlier.35, 36
A matricellular protein, osteopontin, is expressed in response to mechanical stress and similar stimuli, integrates the inter-extracellular matrix signal transduction network of component cells, and maintains efficient contractility through quantitative and qualitative control of extracellular matrix proteins.37 In addition, osteopontin is re-expressed in the process of tissue damage and promotes myocardial remodeling through transforming growth factor-β and MMPs.37 In tendon fibroblasts, MMP-13 expression can be downregulated by exogenous osteopontin.38 Accordingly, it is likely that increased expression of osteopontin in the hippocampal vessels of SHRSP rats may play a role in repair of BBB-damaged vessels with increased expression of MMP-13. Further studies are needed to clarify what accelerates the expression of MMP-13 or osteopontin in BBB-damage vessels. It remains to be clarified why only MMP-13 expression is increased in the BBB-damaged vessels of SHRSP rats among MMPs. It may be due to correlation to osteopontin.38 In addition, as angiotensin II and icotinamide adenine dinucleotide phosphate oxidase play a role in hypertensive brain injury of SHRSP,39 these may be related to hypertensive BBB damage.
Conflict of interest
The authors declare no conflict of interest.
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Acknowledgements
We thank M Kawauchi for technical assistance and A Kimura for editorial assistance. This research was supported by a budget from the Ministry of Education, Culture, Sports, Science and Technology of Japan and a budget for priority research from Faculty of Medicine, Kagawa University, 2008.
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Ueno, M., Wu, B., Nishiyama, A. et al. The expression of matrix metalloproteinase-13 is increased in vessels with blood–brain barrier impairment in a stroke-prone hypertensive model. Hypertens Res 32, 332–338 (2009). https://doi.org/10.1038/hr.2009.26
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DOI: https://doi.org/10.1038/hr.2009.26
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