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Effective population size in ecology and evolution

Effective population size (Ne) is one of the most important parameter in population genetics and conservation biology. It translates census sizes of a real population into the size of an idealized population showing the same rate of loss of genetic diversity as the real population under study. Several conceptually different types of Ne can be distinguished, but the most commonly used ones are those based on the loss of genetic diversity through inbreeding (inbreeding Ne) and through genetic drift (variance Ne). Often, these two differ considerably, as do census and effective sizes, although, contrary to what is taught in many introductory courses and textbooks, Ne is not always lower than census size (Braude and Templeton, 2009, see also Nunney in this issue). Inbreeding and variance Ne particularly differ when demography changes. However, these relationships are not all that well understood yet, and require further theoretical and empirical attention.

Many recent developments in theory, modeling and statistics as well as in molecular biology have sparked new interest in prediction and estimation of Ne and have led to a number of papers documenting recent advancements in the field. This special issue comprises a collection of papers that tackle various theoretical and applied aspects concerning the estimation of Ne under simulated conditions and in natural situations. Contributions were chosen to provide a balanced overview of the topic by summarizing the state of the art (Wang et al. in this issue), providing new developments in theory, improvements in estimation methods, especially in terms of applicability to genome-wide data (Hollenbeck et al.; Jones et al.; Nunney; Waples et al.; Waples, all in this issue), and some case studies employing currently available methods (Coscia et al.; Cousseau et al.; Ferchaud et al.; Laikre et al.; Mueller et al., all in this issue). Although not being comprehensive, the special issue hopes to provide a good overview of the current status of the field as well as providing new advancements.

Wang et al. present a review across the discipline, focusing on the distinction of prediction and estimation methods of Ne. The authors provide a general introduction into the field and describe the theoretical background of current prediction and estimation methods in depth, serving as an introduction to the topic.

The next five papers provide technical and theoretical advancements in estimation methods. The contributions of this part are focusing on new methods addressing specific problems in estimating Ne under natural conditions, several specifically on the linkage disequilibrium (LD) method. The LD method is currently the most used as it allows robust estimation of Ne from samples taken at only a single point in time. One of the big challenges is to adjust these methods for estimating Ne to genomic data, which are often available as Single Nucleotide Polymorphisms (SNPs). Estimating Ne from SNPs is the focus of Waples et al., who adopt the LD method to genomic data sets. The problem with such data is physical linkage of some of the loci, which may bias estimates downwardly. The authors used simulations to show that linkage does not overall increase with higher numbers of loci. Nevertheless, some loci inevitably show strong linkage. The authors present a method to reduce this bias by taking chromosome number and length into account. Yet, the precision of estimates based on SNPs is lower than expected. Jones et al. tackle the problem of reliability of confidence intervals (CIs). They provide two new methods to generate CIs and compare them with previous parametric and jackknife techniques. The new methods are specifically applicable when using large numbers of genomic SNPs and hence represent valuable alternatives for genome-wide data sets. Hollenbeck et al. also use the LD method, but employ it to test for recent changes in Ne using SNP markers. The authors test their new approach on an empirical data set of marine fish in which a recent decline is known to have occurred. They show that even small and recent changes can be detected and thus provide a valuable new tool for conservation studies. A more specific problem with estimating Ne is addressed by Nunney, whose paper clarifies the distinction of Ne and the effective size of a population’s neighborhood (Nn) and also investigates the effects of Nn on Ne using simulations. Nn is primarily based on the dispersal characteristics of a species and is independent of Ne. Simulations show that the effects of Nn on Ne can be substantial when sampling is temporal; the author provides an optimal sampling scheme to reduce such bias and suggests that low effective to census size ratios (Ne/N) may be a result of the bias. In addition to genetic methods, Ne can also be inferred from life history traits. The contribution by Waples reiterates the importance of considering life history traits, specifically those related to adult mortality, and discusses problems with estimating Ne when generations overlap. The author implements life history parameters related to adult mortality in predictive equations and tests these on an empirical data set of 63 animal and plant species. He suggests that a comparison of non-genetic and genetic estimates of Ne may provide valuable information to interpret such estimates in the future.

The third block of papers presents applied studies, where diverse methods of Ne estimation are employed in different contexts and under different natural settings. Ferchaud et al. investigate and compare Ne, the number of breeders (Nb) and the census size (Nc) in Atlantic salmon. They use temporal genetic data to develop models for predicting Nc from Nb. The authors find a high variance in the predictive power of the model and conclud that thorough calibration is necessary before using Nb in conservation studies. Coscia et al. use a different fish system, two species of sea breams with different breeding systems, to investigate the influence of sex change on the estimation of Ne. The authors find that gonochoristic species have lower contemporary Ne. Yet, this difference was not found across historical time scales. The authors conclude that sex change may influence Ne differently over varying time frames, but that this variance may also depend on the genetic markers used to infer demographic parameters. Cousseau et al. use historic and contemporary samples to test for temporal shifts of genetic diversity and Ne in a recently collapsed house sparrow population in the Netherlands. They find no change in genetic diversity before and after the collapse, but detect an increase in differentiation, suggesting that levels of gene flow have declined. Further, Ne was found to be reduced. The study unravels genetic signatures supporting the decline of house sparrows in Europe and underlines the value of historical samples in conservation genetic studies (Habel et al., 2014). Mueller et al. use a large data set of a long-term monitored buzzard population to evaluate single-sample and temporal methods of estimating Ne. The monitoring data further allow the authors to compare Ne and Nc estimates across multiple years. Temporal and single-sample estimators yield largely congruent results. Ne and Nc ratios, however, are highly variable; both Ne and Nc strongly increase during the study period. The study, similar to the sparrow example, clearly shows the high value and importance of long-term monitoring and the potential for misleading results when only a single point in time is considered. In the final paper, Laikre et al. present a conservation study investigating the meta-population size of the Scandinavian wolf population based on empirically estimated demographic parameters. They find the currently realized Ne of the species in the region to be much below conservation targets. Conservation goals can only be reached by strongly increasing Ne and/or increasing immigration from larger Russian populations, as long as gene flow within the meta-population remains high.

The special issue covers a variety of topics from theoretical to applied questions related to Ne, points to the direction in which research currently heads and highlights gaps that need to be filled in the future. It is quite clear that new technologies, especially in terms of data generation (next generation sequencing) and also analytical methods, will enrich not only the estimation of Ne, but also the whole field of population and conservation genetics. We hope this special issue will spark discussion and encourage new research.

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Acknowledgements

We thank all the authors for their contributions. Special thanks also go to the reviewers who helped to guarantee the high scientific standard of the articles. We also want to express our gratitude to Mike Bruford and Mafalda Costa for the opportunity to edit this special issue and their help in composing it.

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Husemann, M., Zachos, F., Paxton, R. et al. Effective population size in ecology and evolution. Heredity 117, 191–192 (2016). https://doi.org/10.1038/hdy.2016.75

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