Abstract
Hybrids carrying the wild type allele of zygotic hybrid rescue (zhr) of Drosophila melanogaster and the maternal cytoplasm of its sibling species (D. simulans, D. mauritiana or D. sechellia) are embryonic lethal, irrespective of their sex. The zhr gene has been localized in the X heterochromatin, slightly distal to the In(1)sc8 breakpoint, a location where highly repetitive satellite DNA exists. A free duplication minichromosome, Dp(1;f)1162, which is derived from the In(1)sc8 chromosome by a massive interstitial deletion has a significant, but not complete, effect on the hybrid lethality. In order to examine the organization of zhr cytogenetically, we synthesized four deletions from Dp(1;f)1162 by γ-ray irradiation. Three of them, Dp(1;f)1162S2, Dp(1;f)1162S3, and Dp(l;f)1162S5, showed a hybrid lethal effect not significantly different from Dp(1;f)1162, but one, Dp(1;f)1162S4, showed a significantly weaker hybrid lethal effect. Interestingly, hybrids were completely lethal when two copies of Dp(1;f)1162S4 were present. That the lethal effect is proportional to the number of the minichromosomes supports the model that a class of repeated sequences around the Dp(1;f)1162S4 breakpoint may be involved in the zhr gene activity. In the future, the mini-chromosome could be useful in the cloning and characterization of this reproductive isolation gene.
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References
Carmena, M, and González, C. 1995. Transposable elements map in a conserved pattern of distribution extending from beta-heterochromatin to centromeres in Drosophila melanogaster. Chromosoma, 103, 676–684.
David, J R, Lemeunier, F, Tsacas, L, and Bocquet, C. 1974. Hybridation d'une nouvelle espèce, Drosophila mauritiana avec D. melanogaster et D. simulans. Ann Genet, 17, 235–241.
Dobzhansky, T H. 1970. Genetics of the Evolutionary Process. Columbia University Press, New York.
Hadorn, E. 1961. Zur Autonomie und Phasenspecifität der Letalität von Bastarden zwischen Drosophila melanogaster und Drosophila simulans. Rev Suisse Zool, 68, 197–207.
Haldane, J B S. 1922. Sex ratio and unisexual sterility in hybrid animals. J Genet, 12, 101–109.
Hutter, P, and Ashburner, M. 1987. Genetic rescue of inviable hybrids between Drosophila melanogaster and its sibling species. Nature, 327, 331–333.
Hutter, P, and Karch, F. 1994. Molecular analysis of a candidate gene for the reproductive isolation between sibling species of Drosophila. Experientia, 50, 749–762.
Hutter, P, Roote, J, and Ashburner, M. 1990. A genetic basis for the inviability of hybrids between sibling species of Drosophila. Genetics, 124, 909–920.
Lachaise, D, David, J R, Lemeunier, F, Tsacas, L, and Ashburner, M. 1986. The reproductive relationships of Drosophila sechellia with D. mauritiana, D. simulans, and D. melanogaster from the Afrotropical region. Evolution, 40, 262–271.
Le, M-H, Duricka, D, and Karpen, G H. 1995. Islands Of complex DNA are widespread in Drosophila centric heterochromatin. Genetics, 141, 283–303.
Lindsley, D L, and Zimm, G G. 1992. The Genome of Drosophila melanogaster. Academic Press, San Diego.
Mayr, E. 1963. Animal Species and Evolution, Belknap, Cambridge. MA.
Orr, H A. 1993. Haldane's rule has multiple genetic causes. Nature, 361, 532–533.
Park, H-S, and Yamamoto, M-T. 1993. Synthesis of free X duplications carrying a specific region of the centromeric heterochromatin in Drosophila melanogaster. Jap J Genet, 68, 83–95.
Sawamura, K. 1996. Maternal effect as a cause of exceptions for Haldane's rule. Genetics, 143, 609–611.
Sawamura, K, and Yamamoto, M-T. 1993. Cytogenetical localization of Zygotic hybrid rescue (Zhr), a Drosophila melanogaster gene that rescues interspecific hybrids from embryonic lethality. Mol Gen Genet, 239, 441–449.
Sawamura, K, Taira, T, and Watanabe, T K. 1993a. Hybrid lethal systems in the Drosophila melanogaster species complex. I. The maternal hybrid rescue (mhr) gene of Drosophila simulans. Genetics, 133, 299–305.
Sawamura, K, Watanabe, T K, and Yamamoto, M-T. 1993b. Hybrid lethal systems in the Drosophila melanogaster species complex. Genetica, 88, 175–185.
Sawamura, K, Yamamoto, M T, and Watanabe, T K. 1993c. Hybrid lethal systems in the Drosophila melanogaster species complex. II. The Zygotic hybrid rescue (Zhr) gene of D. melanogaster. Genetics, 133, 307–313.
Sawamura, K, Fujita, A, Yokoyama, R, Taira, T, Inoue, Y H, Park, H S, and Yamamoto, M T. 1995. Molecular and genetic dissection of a reproductive isolation gene, zygotic hybrid rescue, of Drosophila melanogaster. Jap J Genet, 70, 223–232.
Spofford, J B. 1976. Position-effect variegation in Drosophila. In: Ashburner, M. and Novitski, E. (eds) The Genetics and Biology of Drosophila, 1c, pp. 955–1018.Academic Press, London.
Sturtevant, A H. 1920. Genetic studies on Drosophila simulans. I. Introduction. Hybrids with Drosophila melanogaster. Genetics, 5, 488–500.
Sturtevant, A H. 1929. The genetics of Drosophila simulans. Carnegie Inst Wash Publ, 399, 1–62.
Takamura, T, and Watanabe, T K. 1980. Further studies on the Lethal hybrid rescue (Lhr) gene of Drosophila simulans. Jap J Genet, 55, 405–408.
Watanabe, T K. 1979. A gene that rescues the lethal hybrids between Drosophila melanogaster and D. simulans. Jap J Genet, 54, 325–331.
Wu, C-I, and Davis, A W. 1993. Evolution of postmating reproductive isolation: the composite nature of Haldane's rule and genetic bases. Am Nat, 142, 187–212.
Wu, C I, and Palopoli, M F. 1994. Genetics of postmating reproductive isolation in animals. Ann Rev Genet, 28, 283–308.
Yamamoto, M T. 1992. Inviability of hybrids between D. melanogaster and D. simulans results from the absence of simulans X not the presence of simulans Y chromosome. Genetica, 87, 151–158.
Yamamoto, M, and Miklos, G L G. 1977. Genetic dissection of heterochromatin in Drosophila: the role of basal X heterochromatin in meiotic sex chromosome behaviour. Chromosoma, 60, 283–296.
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Sawamura, K., Yamamoto, MT. Characterization of a reproductive isolation gene, zygotic hybrid rescue, of Drosophila melanogaster by using minichromosomes. Heredity 79, 97–103 (1997). https://doi.org/10.1038/hdy.1997.127
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DOI: https://doi.org/10.1038/hdy.1997.127
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