Over the last few decades, discovery in immunology has been catalyzed by the ability of flow cytometry to produce population statistics out of individual cells, leading to better understanding of cell biology and heterogeneity. Continued innovation has now enabled a powerful, image-based, spectral flow cytometry technology capable of providing unprecedented insights at the single-cell level.
Combining these advances with genomics-based approaches such as single-cell multiomics now offers the ability to probe the proteome, transcriptome and epigenome together at the single-cell level, offering the potential to unravel rare insights otherwise left uncovered with current approaches.
This webcast will present an integrative approach using real-time imaging, spectral flow cytometry and Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq) to perform high-dimensional, multimodal analyses of human T regulatory (Treg) cells. This combinatorial approach reveals different subsets of Tregs while uncovering distinct, heterogeneous proteomic and genomic signatures associated with naïve, activated, and memory Treg cell populations. This approach now offers a novel, multimodal tool set with which to drive the next generation of immunology research.
Learn about:
• Combining spectral, image-based cell sorting and single-cell multiomics to characterize immune populations of interest
• Potential applications for combining RNA plus protein (CITE-seq) in single-cell immunology
• Advanced real-time imaging, spectral flow cytometry (RTI-SFC) technology for cell sorting and downstream applications
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This webcast has been produced by BD Biosciences, who retails sole responsibility for content. About this content.
Speaker
Robert Balderas, Vice President Biological Sciences, BD Biosciences
With 40 years of leadership experience in academia and biotech, Dr. Robert (Bob) Balderas is an expert in immunology and flow cytometry. He has been on the editorial board of Clinical Proteomics, led partnerships with CANVAC and the NCI, and served on several important boards such as the HCMD Council, the UCSD Industrial Liaison Program, the UCSD School of Biological Sciences Dean’s Council, FOCIS, Inflamex, the Oz Single Cell Consortium, the Human Single Cell Germany Consortium (Lifetime), and the UCSD Jacobs School of Engineering. He also led the NIH/BDB Symphony CRADA initiative.
Moderator
Sarah Hiddleston, Nature Research Custom Media
Sarah Hiddleston is a freelance journalist who has worked with Nature Research Custom Media since 2015. Previously, Sarah worked for a decade in Madras (Chennai), India, specialising in health, pharmaceutical and environmental stories. Sarah holds an MA in Investigative Journalism from City University London, an MSc in Political Theory from the London School of Economics, and an undergraduate degree in History from the University of Cambridge, UK.
