Abstract
The aim of this study was to evaluate an engineered nanostructure to silence five important oncogenes, including BAG1, MDM2, Bcl-2, BIRC5 (survivin) and XIAP, in acute myeloid leukemia subtype 2 (AML-M2). The smart nanostructures were functionalized gold nanoparticles (FGNs) containing five antisense oligonucleotides (AOs) and one anti-CD33(+)/CD34(+) aptamer. First, the best AO for each gene was selected with the OligoWalk online software, and then different arrangements of AOs were evaluated with the RNAstructure software. Thereafter, naked gold nanoparticles (NGNs) were synthesized by the reaction of 1000 mm HAuCl4 with 10 μg ml−1 ascorbic acid. Next, five AOs and one anti-CD33(+)/CD34(+) aptamer were attached to NGNs through serial reactions. Later, 5 ml of heparinized blood samples from five AML-M2 patients were prepared, cancerous cells were isolated and then incubated with three concentrations (75, 150 and 300 μg ml−1) each of FGNs, NGNs, gold nanoparticles functionalized with scrambled oligonucleotides (GNFSONs) and doxorubicin. Finally, cell death percentage and gene expressions were measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and real-time PCR, respectively. This study showed that FGNs and doxorubicin led to more cell death compared with NGNs and GNFSONs (P<0.05). Interestingly, all concentrations of FGNs led to a decrease in gene expression. As an important finding, although all concentrations of doxorubicin could also inhibit the expression of genes, FGNs had more effect (P<0.05). Moreover, both NGNs and GNFSONs could silence all genes only at a concentration of 300 μg ml−1. For BCL2 and XIAP, a dose-dependent pattern was observed, but there was no similar pattern for others.
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References
Hope KJ, Jin L, Dick JE . Acute myeloid leukemia originates from a hierarchy of leukemic stem cell classes that differ in self-renewal capacity. Nat Immunol 2004; 5: 738–743.
Fröhling S, Scholl C, Gilliland DG, Levine RL . Genetics of myeloid malignancies: pathogenetic and clinical implications. J Clin Oncol 2005; 23: 6285–6295.
Gong J, Yu J, Lin H, Zhang X, Yin X, Xiao Z et al. The role, mechanism and potentially therapeutic application of microRNA-29 family in acute myeloid leukemia. Cell Death Differ 2014; 21: 100–112.
Mancini E, De Martino L, Malova H, De Feo V . Chemical composition and biological activities of the essential oil from Calamintha nepeta plants from the wild in southern Italy. Nat Product Commun 2013; 8: 139–142.
Moustafa AE-RA, El-Azeem HA, Omran MA, Nasr SA, Nabi IMA, Teleb ZA . Biochemical effect of Nepeta septemcrenata growing in South Sinai, Egypt. Am J Ethnomed 2015; 2: 157–168.
Tallman MS, Gilliland DG, Rowe JM . Drug therapy for acute myeloid leukemia. Blood 2005; 106: 1154–1163.
Tacar O, Sriamornsak P, Dass CR . Doxorubicin: an update on anticancer molecular action, toxicity and novel drug delivery systems. J Pharm Pharmacol 2013; 65: 157–170.
Feng S-S . Nanoparticles of biodegradable polymers for new-concept chemotherapy. Expert Rev Med Dev 2014; 1: 115–125.
Monsuez J-J, Charniot J-C, Vignat N, Artigou J-Y . Cardiac side-effects of cancer chemotherapy. Int J Cardiol 2010; 144: 3–15.
Ju J, Wang N, Wang X, Chen F . A novel all-trans retinoic acid derivative inhibits proliferation and induces differentiation of human gastric carcinoma xenografts via up-regulating retinoic acid receptor β. Am J Transl Res 2015; 7: 856.
Liu Q, Jin C, Wang Y, Fang X, Zhang X, Chen Z et al. Aptamer-conjugated nanomaterials for specific cancer cell recognition and targeted cancer therapy. NPG Asia Mater 2014; 6: e95.
Kamaly N, Swami A, Wagner R, Farokhzad OC . Nanomedicines for Diagnosis and Treatment of Prostate Cancer, Imaging and Focal Therapy of Early Prostate Cancer. Springer: Berlin, Gemany, 2013, pp 203–217.
Estey E, Levine RL, Löwenberg B . Current challenges in clinical development of 'targeted therapies': the case of acute myeloid leukemia. Blood 2015; 125: 2461–2466.
Farokhzad OC, Jon S, Khademhosseini A, Tran T-NT, LaVan DA, Langer R . Nanoparticle–aptamer bioconjugates a new approach for targeting prostate cancer cells. Cancer Res 2004; 64: 7668–7672.
Vinores S . Technology evaluation: pegaptanib, Eyetech/Pfizer. Curr Opin Mol Ther 2003; 5: 673–679.
McManus MT, Sharp PA . Gene silencing in mammals by small interfering RNAs. Nat Rev Genet 2002; 3: 737–747.
Dykxhoorn DM, Lieberman J . The silent revolution: RNA interference as basic biology, research tool, and therapeutic. Annu Rev Med 2005; 56: 401–423.
Brigger I, Dubernet C, Couvreur P . Nanoparticles in cancer therapy and diagnosis. Adv Drug Deliv Rev 2012; 64: 24–36.
Chiannilkulchai N, Driouich Z, Benoit J, Parodi A, Couvreur P . Doxorubicin-loaded nanoparticles: increased efficiency in murine hepatic metastases. Select Cancer Ther 1989; 5: 1–11.
Steiniger SC, Kreuter J, Khalansky AS, Skidan IN, Bobruskin AI, Smirnova ZS et al. Chemotherapy of glioblastoma in rats using doxorubicin‐loaded nanoparticles. Int J Cancer 2004; 109: 759–767.
Byeon HJ, Lee S, Min SY, Lee ES, Shin BS, Choi H-G et al. Doxorubicin-loaded nanoparticles consisted of cationic- and mannose-modified-albumins for dual-targeting in brain tumors. J Control Rel 2016; 225: 301–313.
Jebali A, Anvari-Tafti MH . Hybridization of different antisense oligonucleotides on the surface of gold nanoparticles to silence zinc metalloproteinase gene after uptake by Leishmania major. Colloids Surf B 2015; 129: 107–113.
Almeida JPM, Figueroa ER, Drezek RA . Gold nanoparticle mediated cancer immunotherapy. Nanomed Nanotechnol Med 2014; 10: 503–514.
Khan Z, Singh T, Hussain JI, Hashmi AA . Au (III)–CTAB reduction by ascorbic acid: preparation and characterization of gold nanoparticles. Colloids Surfaces B 2013; 104: 11–17.
Ocana A, Perez-Peña J, Serrano-Heras G, Corrales-Sanchez V, Montero J, Gascón-Escribano M et al. Abstract P3-14-11: gene-expression analyses identify altered transcription factors and supports the antitumor activity of novel bromodomain inhibitors in triple negative breast cancer. Cancer Res 2016; 76: P3-14-11–P13-14-11.
Kole R, Krainer AR, Altman S . RNA therapeutics: beyond RNA interference and antisense oligonucleotides. Nat Rev Drug Discov 2012; 11: 125–140.
Wilson RC, Doudna JA . Molecular mechanisms of RNA interference. Annu Rev Biophys 2013; 42: 217–239.
Stewart AJ, Canitrot Y, Baracchini E, Dean NM, Deeley RG, Cole SP . Reduction of expression of the multidrug resistance protein (MRP) in human tumor cells by antisense phosphorothioate oligonucleotides. Biochem Pharmacol 1996; 51: 461–469.
Zhang S, Wang G, Zhu P, Liang J, Xu Y, Peng M et al. [Screening and structure analysis of nucleic acid aptamers binding to surface of CD33 (+)/CD34 (+) cells from patients with acute myeloid leukemia subtype M2]. J Exp Hematol Chin Assoc Pathophysiol 2011; 19: 561–565.
Xia B, Tian C, Guo S, Zhang L, Zhao D, Qu F et al. c-Myc plays part in drug resistance mediated by bone marrow stromal cells in acute myeloid leukemia. Leukemia Res 2015; 39: 92–99.
Mohammadi M, Salmasi Z, Hashemi M, Mosaffa F, Abnous K, Ramezani M . Single-walled carbon nanotubes functionalized with aptamer and piperazine–polyethylenimine derivative for targeted siRNA delivery into breast cancer cells. Int J Pharm 2015; 485: 50–60.
Bagalkot V, Farokhzad OC, Langer R, Jon S . An aptamer–doxorubicin physical conjugate as a novel targeted drug‐delivery platform. Angew Chem Int Ed 2006; 45: 8149–8152.
Bafghi AF, Jebali A, Daliri K . Silica nanowire conjugated with loop-shaped oligonucleotides: a new structure to silence cysteine proteinase gene in Leishmania tropica. Colloids Surf B 2015; 136: 323–328.
Acknowledgements
This article was extracted from the PhD thesis of Mohammad Ali Zaimy, cosupervised by Dr. J Tavakkoly-Bazzaz and Dr A Jebali. This research was financially supported by the School of Medicine, Tehran University of Medical Sciences, Tehran, Iran (grant number: 31680).
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Zaimy, M., Jebali, A., Bazrafshan, B. et al. Coinhibition of overexpressed genes in acute myeloid leukemia subtype M2 by gold nanoparticles functionalized with five antisense oligonucleotides and one anti-CD33(+)/CD34(+) aptamer. Cancer Gene Ther 23, 315–320 (2016). https://doi.org/10.1038/cgt.2016.33
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DOI: https://doi.org/10.1038/cgt.2016.33
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