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Allografting

Association of IL6 and CCL2 gene polymorphisms with the outcome of allogeneic haematopoietic stem cell transplantation

Bone Marrow Transplantation volume 44, pages 227–235 (2009)Cite this article

Abstract

Various polymorphisms of non-HLA genes have recently been investigated as candidate risk factors in allogeneic haematopoietic SCT (aHSCT). Our study aimed at exploring possible associations of IL6 and CCL2 single nucleotide polymorphisms (SNP) with aHSCT outcome. A total of 166 HLA-identical aHSCT pairs recruited in were genotyped for IL6 −174 G/C, IL6 −597 G/A, CCL2 −2518 A/G and CCL2 −2076 A/T SNPs by PCR with sequence-specific primers (PCR-SSP). The association between IL6 −174 GG genotype and increased risk of acute GVHD was found in whole study group (P=0.03) and in the subgroup of related aHSCT (P=0.01), association between IL6 −597 GG genotype and the occurrence of acute GVHD was detected only in the related aHSCT pairs (P=0.02). Furthermore, reduction in OS was revealed among recipients possessing IL6 −174*G allele in the group of related aHSCT pairs (P=0.04). Presence of CCL2 −2076 TT genotype was associated with decrease of OS (P=0.04) and increase of TRM (P=0.02) in patients transplanted by related donor. These results, in the context of previous findings, suggest that IL6 gene polymorphisms may be associated with aHSCT outcome, particularly in patients transplanted from a related donor.

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Acknowledgements

Technical assistance of S Zachova, M Lukesova, J Onderkova and A Stahelova is gratefully acknowledged. We thank I Kemperle for collection of clinical data of Slovenian aHSCT pairs. This study was supported by the Research Programme of the Ministry of Education, Youth and Sports No. 6198959205 and the Internal Grant Agency of the Ministry of Health No. NR9099.

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Author notes

  1. Z Ambruzova and F Mrazek: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Immunology, Laboratory of Immunogenomics, Palacky University and University Hospital, Olomouc, Czech Republic

    Z Ambruzova, F Mrazek & M Petrek

  2. Department of Haemato-Oncology, Palacky University and University Hospital, Olomouc, Czech Republic

    L Raida, E Faber & K Indrak

  3. Department of Haematology and Oncology, University Hospital, Pilsen, Czech Republic

    P Jindra

  4. Tissue Typing Centre, Blood Transfusion Centre of Slovenia, Ljubljana, Slovenia

    B Vidan-Jeras

  5. Department of Haematology, University Medical Centre, Ljubljana, Slovenia

    J Pretnar

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  1. Z Ambruzova
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Correspondence to M Petrek.

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Supplementary Information accompanies the paper on Bone Marrow Transplantation website (http://www.nature.com/bmt)

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Ambruzova, Z., Mrazek, F., Raida, L. et al. Association of IL6 and CCL2 gene polymorphisms with the outcome of allogeneic haematopoietic stem cell transplantation. Bone Marrow Transplant 44, 227–235 (2009). https://doi.org/10.1038/bmt.2009.16

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