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Vascular administration of adenoviral vector soaked in absorbable gelatin sponge particles (GSP) prolongs the transgene expression in hepatocytes

Cancer Gene Therapy volume 12, pages 116–121 (2005)Cite this article

Abstract

Transcatheter hepatic arterial chemoembolization using emulsions composed of anticancer agents and gelatin sponges (GS) has been an efficient and safe palliative treatment for inoperable hepatocellular carcinoma (HCC). We employed catheter-mediated left hepatic arterial embolization (CHAE) to increase transduction efficiency of adenoviral vector in canine hepatocytes. The emulsion was prepared by mixing pieces of GSP and adenoviral vectors expressing recombinant β-galactosidase (Ad.LacZ) or human hepatocyte growth factor (Ad.hHGF). After the left hepatic artery was catheterized under angiography, CHAE with Ad.LacZ or Ad.hHGF was performed. Livers were removed and stained for LacZ activity on day 7. The expression pattern of LacZ staining was either scarce or patchy around the central hilum of the hepatic artery, or was homogeneously distributed in whole lobes, depending on whether large or small pieces of GSP were used. Hematological and serum biochemical changes during CHAE exhibited only a few effects. The chronological measurement of serum HGF concentration showed that the duration of transgene expression was greater after CHAE with Ad.hHGF. A similar pattern of transgene expression was observed in a rat model after hepatic arterial embolization with differential doses of Ad.hHGF soaked in GSP. These results suggest that hepatic arterial embolization by transcatheter mediated infusion with a mixture of adenovirus-GSP could be used for human HCC.

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Acknowledgements

This work was supported by Dong-A University Research Funding Program (2001). We thank Professor Duffy for proofreading the manuscript.

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Author notes

  1. Byeong-Ho Park and Jin-Hwa Lee: Both authors contributed equally for this study.

Authors and Affiliations

  1. Department of Radiology, Medical College of Dong-A University, Busan, 602-714, South Korea

    Byeong-Ho Park & Jin-Hwa Lee

  2. Department of Pathology, Medical College of Dong-A University, Busan, 602-714, South Korea

    Jin-Sook Jeong & Seo-Hee Rha

  3. Department of Instrumental Medicine, Medical College of Dong-A University, Busan, 602-714, South Korea

    Seung-Eun Kim & Jae-Seok Kim

  4. MRCMT, Medical College of Dong-A University, Busan, 602-714, South Korea

    Jae-Seok Kim & Tae-Ho Hwang

  5. Department of Clinical Pathology, Medical College of Dong-A University, Busan, 602-714, South Korea

    Jeong-Man Kim

  6. Department of Pharmacology, Medical College of Dong-A University, Busan, 602-714, South Korea

    Tae-Ho Hwang

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  1. Byeong-Ho Park
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Correspondence to Tae-Ho Hwang.

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Park, BH., Lee, JH., Jeong, JS. et al. Vascular administration of adenoviral vector soaked in absorbable gelatin sponge particles (GSP) prolongs the transgene expression in hepatocytes. Cancer Gene Ther 12, 116–121 (2005). https://doi.org/10.1038/sj.cgt.7700744

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