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Evaluation of E1B gene-attenuated replicating adenoviruses for cancer gene therapy

Cancer Gene Therapy volume 9, pages 725–736 (2002)Cite this article

Abstract

Gene-attenuated replication-competent adenoviruses are emerging as a promising new modality for the treatment of cancer. For the aim of improving adenoviral vectors for cancer gene therapy, we have constructed genetically attenuated adenoviral vectors with different combinations of E1B genes and investigated the possibility of enhanced oncolytic and replication effects of these engineered replication-competent adenoviruses. We show here that the cytolytic potency of each gene-attenuated replicating adenovirus differed significantly depending on the presence or deletion of E1B 55 kDa and E1B 19 kDa function. More specifically, among the constructed vectors (Ad-ΔE1B19, Ad-ΔE1B55, Ad-ΔE1B19/55, and Ad-wt), E1B 19 kDa–inactivated adenovirus (Ad-ΔE1B19) was the most potent against all tumor cells tested, inducing the largest-sized plaques and marked CPE. Further, cells infected with either Ad-ΔE1B19 or E1B19/55 kDa–deleted adenovirus (Ad-ΔE1B19/55) showed complete cell lysis with disintegrated cellular structure, whereas cells infected with Ad-wt maintained intact cellular and nuclear membrane with properly structured organelles. TUNEL and DNA fragmentation assay also revealed that the Ad-ΔE1B19 or Ad-ΔE1B19/55 adenovirus-infected cells showed more profound induction of apoptosis in comparison to wild-type adenovirus-infected cells. The presence of E1B 55 kDa gene was required for efficient viral replication and deletion of E1B 19 kDa function in replicating adenovirus-induced apoptosis, leading to increased cytopathic effects. Moreover, Ad-ΔE1B19 adenovirus showed a better antitumor effect than other E1B-attenuated adenoviruses. Taken together, the replicating adenoviruses deleted in E1B 19 kDa function may serve as an improved vector for anticancer gene therapy in combination with apoptosis-inducing modalities such as chemotherapeutic agents and radiation therapy.

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Acknowledgements

We would like to thank all of our colleagues at Yosei Cancer Center, Seoul, Korea who have contributed to these studies. We thank J-H Son for his valueable contribution. This work was supported by grants from Ministry of Health & Welfare, Republic of Korea (HMP-01-PJI-PG3-20800-0115, C-O Yun) and Ministry of Commerce Industry and Energy, Republic of Korea (N03-990-5411-01-1-3, J-H Kim). Jaesung Kim is a graduate student sponsored by Brain Korea 21 Project for Medical Science, Yonsei University.

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Authors and Affiliations

  1. Institute of Cancer Research, Yonsei Cancer Center, Yonsei University College of Medicine, Seoul, South Korea

    Jaesung Kim, Joo-Hang Kim & Chae-Ok Yun

  2. Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea

    Jae Yong Cho

  3. Department of Pathology, Hallym University College of Medicine, Chunchon-Si, Kangwon-Do, South Korea

    Kyeong Cheon Jung

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Correspondence to Chae-Ok Yun.

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Kim, J., Cho, J., Kim, JH. et al. Evaluation of E1B gene-attenuated replicating adenoviruses for cancer gene therapy. Cancer Gene Ther 9, 725–736 (2002). https://doi.org/10.1038/sj.cgt.7700494

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