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A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10% serum

Cancer Gene Therapy volume 7, pages 513–520 (2000)Cite this article

Abstract

We developed a new cationic lipid suitable for use as a DNA carrier in the presence of 10% sera. The novel compound (abbreviated as Arg-Chol) contains cholesterol and a dipeptide consisting of glycine and sterically protected arginine. The efficiency of reporter gene transfection using liposomes based on this new reagent was compared with that of liposomes made with other cationic derivatives of cholesterol. Lipoplexes formulated with the newly synthesized lipid mediate in vitro transfection of B16(F10) murine melanoma cells in the presence of 10% sera more efficiently than in other cell lines and compared with other cholesterol derivatives studied.

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Authors and Affiliations

  1. Department of Molecular Biology, Center of Oncology - Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland

    Aleksander Sochanik & Stanisław Szala

  2. Department of Molecular Oncogenetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, Ukraine

    Irina Kaida

  3. Institute of Organic Chemistry and Technology, Silesian Technical University, Gliwice, Poland

    Iwona Mitrus & Andrzej Rajca

Authors
  1. Aleksander Sochanik
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  2. Irina Kaida
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  3. Iwona Mitrus
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  4. Andrzej Rajca
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  5. Stanisław Szala
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Sochanik, A., Kaida, I., Mitrus, I. et al. A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10% serum. Cancer Gene Ther 7, 513–520 (2000). https://doi.org/10.1038/sj.cgt.7700167

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  • DOI: https://doi.org/10.1038/sj.cgt.7700167

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