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Biological chemistry

Enzymes in focus

The technique of directed evolution creates thousands of mutant enzymes from a single original. A new approach helps to search for variants that have an increased range of substrates — and can thus be used for organic synthesis.

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Figure 1: View of the enzyme active site used by Reetz et al.1 for pair mutation.


  1. Reetz, M. T., Bocola, M., Carballeira, J. D., Zha, D. & Vogel, A. Angew. Chem. Int. Edn 44, 4192–4196 (2005).

    CAS  Article  Google Scholar 

  2. Park, S. et al. Chem. Biol. 12, 45–54 (2005).

    CAS  Article  Google Scholar 

  3. Hill, C. M., Li, W. -S., Thoden, J. B., Holden, H. M. & Raushel, F. M. J. Am. Chem. Soc. 125, 8990–8991 (2003).

    CAS  Article  Google Scholar 

  4. Whittle, E. & Shanklin, J. J. Biol. Chem. 276, 21500–21505 (2001).

    CAS  Article  Google Scholar 

  5. Koga, Y., Kato, K., Nakano, H. & Yamane, T. J. Mol. Biol. 331, 585–592 (2003).

    CAS  Article  Google Scholar 

  6. Li, Q. -S. et al. Biochim. Biophys. Acta 1545, 114–121 (2001).

    CAS  Article  Google Scholar 

  7. Wells, J. A., Vasser, M. & Powers, D. B. Gene 34, 315–323 (1985).

    CAS  Article  Google Scholar 

  8. Horsman, G. P. et al. Chem. Eur. J. 9, 1933–1939 (2003).

    CAS  Article  Google Scholar 

  9. Morley, K. L. & Kazlauskas, R. J. Trends Biotechnol. 23, 231–237 (2005).

    CAS  Article  Google Scholar 

  10. Liebeton, K. et al. Chem. Biol. 7, 709–715 (2000).

    CAS  Article  Google Scholar 

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Kazlauskas, R. Enzymes in focus. Nature 436, 1096–1097 (2005).

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