Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Biological chemistry

Enzymes in focus

The technique of directed evolution creates thousands of mutant enzymes from a single original. A new approach helps to search for variants that have an increased range of substrates — and can thus be used for organic synthesis.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

Figure 1: View of the enzyme active site used by Reetz et al.1 for pair mutation.

References

  1. Reetz, M. T., Bocola, M., Carballeira, J. D., Zha, D. & Vogel, A. Angew. Chem. Int. Edn 44, 4192–4196 (2005).

    CAS  Article  Google Scholar 

  2. Park, S. et al. Chem. Biol. 12, 45–54 (2005).

    CAS  Article  Google Scholar 

  3. Hill, C. M., Li, W. -S., Thoden, J. B., Holden, H. M. & Raushel, F. M. J. Am. Chem. Soc. 125, 8990–8991 (2003).

    CAS  Article  Google Scholar 

  4. Whittle, E. & Shanklin, J. J. Biol. Chem. 276, 21500–21505 (2001).

    CAS  Article  Google Scholar 

  5. Koga, Y., Kato, K., Nakano, H. & Yamane, T. J. Mol. Biol. 331, 585–592 (2003).

    CAS  Article  Google Scholar 

  6. Li, Q. -S. et al. Biochim. Biophys. Acta 1545, 114–121 (2001).

    CAS  Article  Google Scholar 

  7. Wells, J. A., Vasser, M. & Powers, D. B. Gene 34, 315–323 (1985).

    CAS  Article  Google Scholar 

  8. Horsman, G. P. et al. Chem. Eur. J. 9, 1933–1939 (2003).

    CAS  Article  Google Scholar 

  9. Morley, K. L. & Kazlauskas, R. J. Trends Biotechnol. 23, 231–237 (2005).

    CAS  Article  Google Scholar 

  10. Liebeton, K. et al. Chem. Biol. 7, 709–715 (2000).

    CAS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kazlauskas, R. Enzymes in focus. Nature 436, 1096–1097 (2005). https://doi.org/10.1038/4361096a

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/4361096a

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing