The technique of directed evolution creates thousands of mutant enzymes from a single original. A new approach helps to search for variants that have an increased range of substrates — and can thus be used for organic synthesis.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Reetz, M. T., Bocola, M., Carballeira, J. D., Zha, D. & Vogel, A. Angew. Chem. Int. Edn 44, 4192–4196 (2005).
Park, S. et al. Chem. Biol. 12, 45–54 (2005).
Hill, C. M., Li, W. -S., Thoden, J. B., Holden, H. M. & Raushel, F. M. J. Am. Chem. Soc. 125, 8990–8991 (2003).
Whittle, E. & Shanklin, J. J. Biol. Chem. 276, 21500–21505 (2001).
Koga, Y., Kato, K., Nakano, H. & Yamane, T. J. Mol. Biol. 331, 585–592 (2003).
Li, Q. -S. et al. Biochim. Biophys. Acta 1545, 114–121 (2001).
Wells, J. A., Vasser, M. & Powers, D. B. Gene 34, 315–323 (1985).
Horsman, G. P. et al. Chem. Eur. J. 9, 1933–1939 (2003).
Morley, K. L. & Kazlauskas, R. J. Trends Biotechnol. 23, 231–237 (2005).
Liebeton, K. et al. Chem. Biol. 7, 709–715 (2000).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kazlauskas, R. Enzymes in focus. Nature 436, 1096–1097 (2005). https://doi.org/10.1038/4361096a
Published:
Issue Date:
DOI: https://doi.org/10.1038/4361096a
