Abstract
RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Elbashir, S. M. et al. Nature 411, 494–498 (2001).
Zhang, G., Budker, V. & Wolff, J. A. Hum. Gene Therapy 10, 1735–1737 (1999).
Liu, F., Song, Y. & Liu, D. Gene Therapy 6, 1258–1266 (1999).
Chang, J., Sigal, L. J., Lerro, A. & Taylor, J. J. Virol. 75, 3469–3473 (2001).
Contag, C. H. et al. Photochem. Photobiol. 66, 523–531 (1997).
Yant, S. R. et al. Nature Genet. 25, 35–41 (2000).
Bitko, V. & Barik, S. BMC Microbiol. 1, 34 (2001).
Paddison, P. J., Caudy, A. A., Bernstein, E., Hannon, G. J. & Conklin, D. S. Genes Dev. 16, 948–958 (2002).
Tuschl, T. Nature Biotechnol. 20, 446–448 (2002).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Rights and permissions
About this article
Cite this article
McCaffrey, A., Meuse, L., Pham, TT. et al. RNA interference in adult mice. Nature 418, 38–39 (2002). https://doi.org/10.1038/418038a
Issue Date:
DOI: https://doi.org/10.1038/418038a
This article is cited by
-
NKG2D knockdown improves hypoxic-ischemic brain damage by inhibiting neuroinflammation in neonatal mice
Scientific Reports (2024)
-
RNA therapy: rich history, various applications and unlimited future prospects
Experimental & Molecular Medicine (2022)
-
Effects on the STAT3-shRNA in Non-Small-Cell Lung Cancer Therapy: Design, Induction of Apoptosis, and Conjugation with Chitosan-Based Gene Vectors
Journal of Ocean University of China (2021)
-
Pharma’s roller-coaster relationship with RNA therapies
Nature (2019)
-
RNA therapies explained
Nature (2019)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.