Abstract
IN fission yeast, Cdc2 kinase has both positive and negative roles in regulating DNA replication, being first necessary for the transition from Gl to S phase and later required to prevent the re-initiation of DNA replication during G21–3. We report here that Cdc2 interacts with Orp2, a protein similar to the Orc2 replication factor subunit of Saccharomyces cerevisiae origin recognition complex (ORC). ORC binds chromosomal origins and is essential for chromosomal replication initiation4–6. Fission yeast Orp2 is required for DNA replication and interacts with the rate-limiting replication activator CdclS. Cells lacking Orp2 undergo aberrant mitosis, indicating that Orp2 is involved in generating a checkpoint signal. These findings suggest that ORC functions are conserved among eukaryotes and provide evidence that Cdc2 controls DNA replication initiation by acting directly at chromosomal origins.
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References
Nurse, P. & Bisset, Y. Nature 292, 558–560 (1981).
Broek, D., Bartlett, R., Crawford, K. & Nurse, P. Nature 349, 388–393 (1991).
Hayles, J., Fisher, D., Woollard, A. & Nurse, P. Cell 78, 813–822 (1994).
Micklem, G., Rowley, A., Harwood, J., Nasmyth, K. & Diffley, J. F. X. Nature 366, 87–89 (1993).
Foss, M., McNally, F. J., Laurenson, P. & Rine, J. Science 262, 1838–1844 (1993).
Bell, S. P., Kobayashi, R. & Stillman, B. Science 262, 1844–1849 (1993).
Fields, S. & Song, O. K. Nature 340, 245–246 (1989).
Bell, S. P. & Stillman, B. Nature 357, 128–134 (1992).
Fox, C. A., Loo, S., Dillin, A. & Rine, J. Genes Dev. 9, 911–924 (1995).
Li, J. J. & Herskowitz, I. Science 262, 1870–1874 (1993).
Loo, S. et al. Molec. Biol. Cell 6, 741–756 (1995).
Liang, C., Weinreich, M. & Stillman, B. Cell 81, 667–676 (1995).
Diffley, J. F. X. & Cocker, J. H. Nature 357, 169–172 (1992).
Diffley, J. F. X., Cocker, J. H., Dowell, S. J. & Rowley, A. Cell 78, 303–316 (1994).
Enoch, T. & Nurse, P. Cell 65, 921–923 (1991).
Al-Khodairy, F. & Carr, A. M. EMBO J. 11, 1343–1350 (1992).
Maundrell, K., Hutchinson, A. & Shall, S. EMBO J. 7, 2203–2209 (1988).
Maundrell, K., Wright, A. P. H., Piper, M. & Shall, S. Nucleic Acids Res. 13, 3711–3722 (1985).
Kelly, T. J. et al. Cell 74, 371–382 (1993).
Nishitani, H. & Nurse, P. Cell 83, 397–405 (1995).
Zhou, C., Huang, S.-H. & Jong, A. Y. J. biol. Chem. 264, 9022–9029 (1989).
Hartwell, L. J. molec. Biol. 104, 803–817 (1976).
Bueno, A. & Russell, P. EMBO J. 11, 2167–2176 (1992).
Durfee, T. et al. Genes Dev. 7, 555–569 (1993).
Maier, E. et al. Nature Genet. 1, 273–277 (1992).
Hoheisel, J. D. et al. Cell 73, 109–120 (1993).
Costello, G., Rodgers, L. & Beach, D. Curr. Genet. 11, 119–125 (1986).
Maundrell, K. Gene 123, 127–130 (1993).
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Leatherwood, J., Lopez-Girona, A. & Russell, P. Interaction of Cdc2 and CdclS with a fission yeast ORC2-like protein. Nature 379, 360–363 (1996). https://doi.org/10.1038/379360a0
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DOI: https://doi.org/10.1038/379360a0
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