Abstract
SKELETAL muscle development involves the formation of multi-nucleated myotubes. This is thought to proceed by the induction of differentiation (acquisition of fusion competence) of myoblast cells, their aggregation, and union of their plasma membranes1á¤-3. Various membrane proteins including N-4,5 and M-cadherins6, N-5,7-9 and V-CAMs10 and integrins10,11 participate in myotube formation, but the molecular mechanisms of muscle cell fusion are poorly understood. Here we report the identification of three new, myoblast-expressed gene products, meltrin-αβ and γ, with homology to both viper haemorrhagic factors12,13 and fertilin (PH-30)14,15, a membrane protein involved in eggá¤-sperm fusion. Meltrin-α, a member of the metalloproteinase/disintegrin protein family, appears to be required for myotube formation. Involvement of a fertilin-related protein in myogenesis suggests that there are common mechanisms in gamete and myoblast fusion.
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References
Yaffe, D. & Feldman, M. Devl Biol. 11, 300–317 (1965).
Mintz, B. & Baker, W. W. Proc. natn. Acad. Sci. U.S.A. 58, 592–598 (1967).
Knudsen, K. A. in Membrane Fusion (eds Wilschut, J. & Hoekstra, D.) 601–602 (Marcel Dekker, 1992).
Knudsen, K. A., Myers, L. & McElwee, S. A. Expl Cell Res. 188, 175–184 (1990).
Mege, R. M. et al. J. Cell Sci. 103, 897–906 (1992).
Donalies, M., Cramer, M., Ringwald, M. & Starzinski-Powitz, A. Proc. natn. Acad. Sci. U.S.A. 88, 8024–8028 (1991).
Knudsen, K. A., McElwee, S. A. & Myers, L. Devl Biol. 138, 159–168 (1990).
Dickson, G., Peck, D., Moore, S. E., Barton, C. H. & Walsh, F. S. Nature 344, 348–351 (1990).
Peck, D. & Walsh, F. S. J. Cell Biol. 123, 1587–1595 (1993).
Rosen, G. D. et al. Cell 69, 1107–1119 (1992).
Menko, A. S. & Boettinger, D. Cell 51, 51–57 (1987).
Takeya, H., Oda, K., Miyata, T., Omori-Sato, T. & Iwanaga, S. J. biol. Chem. 265, 16068–16073 (1990).
Paine, M. J. I., Desmond, H. P., David, R., Theakston, G. & Crampton, J. M. J. biol. Chem. 267, 22869–22876 (1992).
Primakoff, P., Hyatt, H. & Tredick-Kline, J. J. Cell Biol. 104, 141–149 (1987).
Blobel, C. P. et al. Nature 356, 248–252 (1992).
Morrison, T. G. Virus Res. 10, 113–136 (1988).
Lamb, R. A. Virology 197, 1–11 (1993).
Wright, W. E., Sassoon, D. A. & Lin, V. K. Cell 56, 607–617 (1989).
Au, L. C. et al. Biochem. biophys. Res. Commun. 181, 585–593 (1991).
Neeper, M. P. & Jacobson, M. A. Nucleic Acids Res. 18, 4255 (1990).
Blobel, C., Myles, D. G., Primakoff, P. & White, J. M. J. Cell Biol. 111, 69–78 (1990).
Mizushima, S. & Nagata, S. Nucleic Acids Res. 18, 5322 (1990).
Gould, R. J. et al. Proc. Soc. exp. Biol. Med. 195, 168–171 (1990).
Myles, D. G., Kimmel, L. H., Blobel, C. P., White, J. M. & Primakoff, P. Proc. natn. Acad. Sci. U.S.A. 91, 4195–4198 (1994).
Couch, C. & Strittmatter, W. Cell 32, 257–265 (1983).
Yoshida, S. et al. Int. Immun. 2, 585–595 (1990).
Perry, A. C., Jones, R., Barker, P. J. & Hall, L. Biochem. J. 286, 671–675 (1992).
Emi, M. et al. Nature Genet. 5, 151–157 (1993).
Smith, D. B. & Johnson, K. S. Gene 67, 31–40 (1988).
Vaidya, T. B., Rhodes, S. J., Taparowsky, E. J. & Konieczny, S. F. Molec. cell. Biol. 9, 3576–3579 (1989).
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Yagami-Hiromasa, T., Sato, T., Kurisaki, T. et al. A metalloprotease-disintegrin participating in myoblast fusion. Nature 377, 652–656 (1995). https://doi.org/10.1038/377652a0
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DOI: https://doi.org/10.1038/377652a0
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