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SEC21 is a gene required for ER to Golgi protein transport that encodes a subunit of a yeast coatomer

Nature volume 360, pages 603–605 (1992)Cite this article

Abstract

NON-CLATHRIN coated vesicles have been implicated in early steps of intercompartmental transport1–4. A distinct set of coat proteins are peripherally associated with the exterior of purified mammalian intra-Golgi transport vesicles5. The 'coatomer', a cytosolic complex containing a similar subunit composition to and sharing at least one subunit (β-COP) with the coat found on vesicles, has been postulated to be the precursor of this non-clathrin coat6. Here we describe the characterization of SEC21, an essential gene required for protein transport from the endoplasmic reticulum to the Golgi in the yeast Saccharomyces cerevisiae. The 105K product of this gene, Sec21p, participates in a cytosolic complex that we show to be a yeast homologue of the mammalian coatomer. These observations demonstrate that a non-clathrin coat protein plays an essential role in intercompartmental transport.

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Authors and Affiliations

  1. Department of Molecular and Cell Biology and Howard Hughes Medical Institute, 401 Barker Hall, University of California, Berkeley, California, 94720, USA

    Midori Hosobuchi & Randy Schekman

  2. Département de Biologic Cellulaire, Université de Genève, 30 quai Ernest-Ansermet, CH-1211, Genève, 4, Switzerland

    Thomas Kreis

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  1. Midori Hosobuchi
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  2. Thomas Kreis
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Hosobuchi, M., Kreis, T. & Schekman, R. SEC21 is a gene required for ER to Golgi protein transport that encodes a subunit of a yeast coatomer. Nature 360, 603–605 (1992). https://doi.org/10.1038/360603a0

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