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Activation of BPV-1 replication in vitro by the transcription factor E2

Nature volume 353, pages 628–632 (1991)Cite this article

Abstract

Soluble extracts from uninfected murine cells supplemented with purified viral E1 and E2 proteins support the replication of exogenously added papilloma virus DNA. The E2 transactivator stimulates the binding of the E1 replication protein to the minimal origin of replication and activates DNA replication. These results support the concept that transcription factors have a direct role in the initiation of DNA replication in eukaryotes by participating in the assembly of a complex at the origin of replication.

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Author notes

  1. Robin Clark: Cetus Corporation, Emeryville, California 94608, USA.

Authors and Affiliations

  1. Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, California, 94720, USA

    Liu Yang, Rong Li, Ian J. Mohr, Robin Clark & Michael R. Botchan

Authors
  1. Liu Yang
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  2. Rong Li
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  3. Ian J. Mohr
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  4. Robin Clark
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  5. Michael R. Botchan
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Yang, L., Li, R., Mohr, I. et al. Activation of BPV-1 replication in vitro by the transcription factor E2. Nature 353, 628–632 (1991). https://doi.org/10.1038/353628a0

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