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Cloning of a human gene encoding the general transcription initiation factor IIB

Naturevolume 352pages689695 (1991) | Download Citation

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Abstract

Transcription factor IIB (TFIIB) has a central role in transcription of class II genes. The purification of the human TFIIB protein and isolation of a complementary DNA encoding TFIIB activity is reported here. The sequence of TFIIB, which seems to be encoded by a single gene, contains a repeated motif, in addition to a motif with similarity to the prokaryotic σ-factors. The recombinant protein expressed in bacteria substituted for all the functions attributed to the human TFIIB protein.

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Author notes

  1. William S. Lane: Harvard Microchemistry Facility, Harvard University, Cambridge, Massachusetts, USA

Affiliations

  1. Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, New Jersey, 08854-5635, USA

    • Ilho Ha
    • , William S. Lane
    •  & Danny Reinberg

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https://doi.org/10.1038/352689a0

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