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Elevated UV-B radiation reduces genome stability in plants

Nature volume 406, pages 98–101 (2000)Cite this article

Abstract

Long-term depletion of the stratospheric ozone layer contributes to an increase in terrestrial solar ultraviolet-B radiation1,2,3. This has deleterious effects on living organisms, such as DNA damage4,5. When exposed to elevated ultraviolet-B radiation (UV-B; 280–315 nm), plants display a wide variety of physiological and morphological responses characterized as acclimation and adaptation6. Here we show, using special sun simulators, that elevated solar UV-B doses increase the frequency of somatic homologous DNA rearrangements in Arabidopsis and tobacco plants. Increases in recombination are accompanied by a strong induction of photolyase and Rad51 gene expression. These genes are putatively involved in major DNA repair pathways, photoreactivation and recombination repair7,8. In mutant Arabidopsis plants that are deficient in photoreactivating ultraviolet-induced cyclobutane pyrimidine dimers, recombination under elevated UV-B regimes greatly exceeds wild-type levels. Our results show that homologous recombination repair pathways might be involved in eliminating UV-B-induced DNA lesions in plants. Thus, increases in terrestrial solar UV-B radiation as forecasted for the early 21st century may affect genome stability in plants.

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Figure 1: Transgenic Arabidopsis thaliana plants (line A11) grown under different daily UV-B doses.
Figure 2: UV-B acclimation responses in Arabidopsis thaliana line A651 grown under different daily UV-B doses.

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Acknowledgements

We thank C. Langebartels, T. Boller, J. Lucht, G. Buchholz, H. Frohnmeyer, P. Crouzet, P. Pelczar, C. Körner and A. Kuttenberger for discussions and comments. We also thank S. Stich, V. Gloeckler and C. Ramos for assistance. The photolyase mutant line (uvr2-1) was obtained from the Arabidopsis Biological Resource Center, Ohio, USA. This work was supported by a fellowship from the Foundation of the Chemical Industry of Basel to G.R. and Novartis Research Foundation.

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Author notes

  1. Holger Puchta

    Present address: IPK, Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466, Gatersleben, Germany

Authors and Affiliations

  1. Friedrich Miescher-Institut, PO Box 2543, Basel, CH-4002, Switzerland

    Gerhard Ries, Holger Puchta & Barbara Hohn

  2. GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg, D-85764, Germany

    Werner Heller, Heinrich Sandermann & Harald K. Seidlitz

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  1. Gerhard Ries
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Correspondence to Gerhard Ries.

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Ries, G., Heller, W., Puchta, H. et al. Elevated UV-B radiation reduces genome stability in plants. Nature 406, 98–101 (2000). https://doi.org/10.1038/35017595

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