Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

RNA editing by cytidine insertion in mitochondria of Physarum polycephalum

Abstract

A COROLLARY of the central dogma of molecular biology is that genetic information passes from DNA to RNA by the continuous synthesis of RNA on a DNA template. The demonstration of RNA editing1 (the specific insertion, deletion or substitution of residues in RNA to create an RNA with a sequence different from its own template) raised the possibility that in some cases not all of the genetic information for a trait resides in the DNA template. Two different types of RNA editing have been identified in mitochondria: insertional editing represented by the extensive insertion (and occasional deletion) of uridine residues in mitochondrial RNAs of the kinetoplastid protozoa2–4 and the substitutional editing represented by the cytidine to uridine substitutions in some plant mitochondria5–7. These editing types have not been shown to be present in the same organism and may have very different mechanisms. RNA editing of both types has been observed in non-mitochondrial systems8–17 but is not as extensive and may involve still different mechanisms. Here we report the discovery of extensive insertional RNA editing in mitochondria from an organism other than a kinetoplastid protozoan. The mitochondrial RNA apparently encoding the α subunit of ATP synthetase in the acellular slime mould, Physarum polycephalum, is edited at 54 sites by cytidine insertion.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

References

  1. Benne, R. et al. Cell 46, 819–826 (1986).

    CAS  Article  Google Scholar 

  2. Benne, R. Biochim. biophys. Acta 1007, 131–139 (1989).

    CAS  Article  Google Scholar 

  3. Simpson, L. & Shaw, J. Cell 57, 355–366 (1989).

    CAS  Article  Google Scholar 

  4. Stuart, K. Parasitol. Today 5, 5–8 (1989).

    CAS  Article  Google Scholar 

  5. Gualberto, J. M., Lammatina, L., Bonnard, G., Weil, J. H. & Grienenberger, J. M. Nature 341, 660–662 (1989).

    ADS  CAS  Article  Google Scholar 

  6. Covello, P. S. & Gray, M. W. Nature 341, 662–666 (1989).

    ADS  CAS  Article  Google Scholar 

  7. Hiesel, R., Wissinger, B., Schuster, W. & Brennicke, A. Science 246, 1632–1634 (1989).

    ADS  CAS  Article  Google Scholar 

  8. Chen, S. H. et al. Science 238, 363–366 (1987).

    ADS  CAS  Article  Google Scholar 

  9. Hospattankar, A. V., Higuchi, K., Law, S. W., Meglin, N. M. & Brewer, H. B. Biochim. Biophys. Res. Commun. 148, 279–285 (1987).

    CAS  Article  Google Scholar 

  10. Powell, L. M. et al. Cell 50, 831–840 (1987).

    CAS  Article  Google Scholar 

  11. Cattaneo, R. et al. Cell 55, 255–265 (1988).

    MathSciNet  CAS  Article  Google Scholar 

  12. Lamb, R. & Dreyfuss, G. Nature 337, 19–20 (1989).

    ADS  CAS  Article  Google Scholar 

  13. Bass, B. L. & Weintraub, H. Cell 55, 1089–1098 (1988).

    CAS  Article  Google Scholar 

  14. Kimelman, D. & Kirschner, M. W. Cell 59, 687–696 (1989).

    CAS  Article  Google Scholar 

  15. Schwer, B. & Stunnenberg, H. G. EMBO J. 7, 1183–1190 (1988).

    CAS  Article  Google Scholar 

  16. Thomas, S. M., Lamb, R. A. & Patterson, R. G. Cell 54, 891–902 (1988).

    CAS  Article  Google Scholar 

  17. Cattaneo, R., Kaelin, K., Baczko, K. & Billeter, M. A. Cell 56, 759–764 (1989).

    CAS  Article  Google Scholar 

  18. Jones, E. J., Mahendran, R., Spottswood, M. R., Yang, Y-C. & Miller, D. L. Curr. Genet. 17, 331–337 (1990).

    CAS  Article  Google Scholar 

  19. Saiki, R. K. et al. Science 239, 487–491 (1988).

    ADS  CAS  Article  Google Scholar 

  20. Bjork, G. R. et al. A. Rev. Biochem. 56, 263–287 (1987).

    CAS  Article  Google Scholar 

  21. Elliott, M. S. & Trewyn, R. W. J. biol. Chem. 259, 2407–2410 (1984).

    CAS  PubMed  Google Scholar 

  22. Farkas, W. R. & Singh, R. D. J. biol. Chem. 248, 7780–7785 (1973).

    CAS  PubMed  Google Scholar 

  23. Howes, N. K. & Farkas, W. R. J. biol. Chem. 253, 9082–9087 (1978).

    CAS  PubMed  Google Scholar 

  24. Okada, N. & Nishimura, S. J. biol. Chem. 254, 3061–3066 (1979).

    CAS  PubMed  Google Scholar 

  25. Okada, N. et al. J. biol. Chem. 254, 3067–3073 (1979).

    CAS  PubMed  Google Scholar 

  26. Blum, B., Bakalara, N. & Simpson, L. Cell 60, 189–198 (1990).

    CAS  Article  Google Scholar 

  27. Mohberg, J. & Rusch, H. P. Expl Cell Res. 66, 305–316 (1971).

    CAS  Article  Google Scholar 

  28. Walker, J. E. et al. J. molec. Biol. 184, 677–701 (1985).

    CAS  Article  Google Scholar 

  29. Braun, C. J. & Levings, C. S. III Pl. Physiol. 79, 571–577 (1985).

    CAS  Article  Google Scholar 

  30. Takeda, M., Chen, W. J., Saltgaber, J. & Douglas, M. J. biol. Chem. 261, 15126–15133 (1986).

    CAS  PubMed  Google Scholar 

  31. Walker, J. E., Saraste, M. & Gay, N. J. Biochim. biophys. Acta 768, 164–200 (1984).

    CAS  Article  Google Scholar 

  32. Tybulewicz, V. L. J., Falk, G. & Walker, J. E. J. molec. Biol. 179, 185–214 (1984).

    CAS  Article  Google Scholar 

  33. McCarn, D. F., Whitaker, R. A., Alam, J., Vrba, J. M. & Curtis, S. E. J. Bact. 170, 3448–3458 (1988).

    CAS  Article  Google Scholar 

  34. Deno, H., Shinozaki, K. & Sugiura, M. Nucleic Acids Res. 11, 2185–2191 (1983).

    CAS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mahendran, R., Spottswood, M. & Miller, D. RNA editing by cytidine insertion in mitochondria of Physarum polycephalum. Nature 349, 434–438 (1991). https://doi.org/10.1038/349434a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/349434a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing