Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Induction of male sterility in plants by a chimaeric ribonuclease gene

Abstract

Chimaeric ribonuclease genes that are expressed in the anthers of transformed tobacco and oilseed rape plants were constructed. Chimaeric ribonuclease gene expression within the anther selectively destroys the tapetal cell layer that surrounds the pollen sac, prevents pollen formation, and leads to male sterility. These nuclear male sterility genes should facilitate the production of hybrid seed in various crop plants.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

References

  1. Esau, K. Anatomy of Seed Plants. 2nd edn (Wiley, 1977).

    Google Scholar 

  2. Kamalay, J. C. & Goldberg, R. B. Cell 19, 935–946 (1980).

    Article  CAS  Google Scholar 

  3. Kamalay, J. C. & Goldberg, R. B. Proc. natn. Acad. Sci. U.S.A. 81, 2801–2805 (1984).

    Article  ADS  CAS  Google Scholar 

  4. Chapman, G. P. Int. Rev. Cytol. 107, 111–125 (1987).

    Article  Google Scholar 

  5. Mascarenhas, J. P. A. Rev. Pl. Physiol. Pl. molec. Biol. 41, 317–338 (1990).

    Article  Google Scholar 

  6. Knox, R. B. Pollen and Allergy (Arnold, London, 1979).

    Google Scholar 

  7. Kaul, M. L. H. Monographs on Theor. and Appl. Genet. Vol. 10 Male Sterility in Higher Plants (Springer, Berlin, 1988).

    Google Scholar 

  8. Goldberg, R. B. Science 240, 1460–1467 (1988).

    Article  ADS  CAS  Google Scholar 

  9. Seurinck, J., Truettner, J. & Goldberg, R. B. Nucleic Acids Res. 18, 3403 (1990).

    Article  CAS  Google Scholar 

  10. Varner, J. & Cassab, G. A. Rev. Pl. Physiol. Pl. molec. Biol. 39, 321–353 (1988).

    Article  Google Scholar 

  11. Condit, C. M. & Meagher, R. B. Nature 323, 178–181 (1986).

    Article  ADS  CAS  Google Scholar 

  12. Keller, B., Schmid, J. & Lamb, C. EMBO J. 8, 1309–1314 (1988).

    Article  Google Scholar 

  13. Jefferson, R. Pl. molec. Biol. Rept 5, 387–405 (1987).

    Article  CAS  Google Scholar 

  14. Quaas, R. et al. Eur. J. biochem. 173, 617–622 (1988).

    Article  CAS  Google Scholar 

  15. Hartley, R. W. J. molec. Biol. 202, 913–915 (1988).

    Article  CAS  Google Scholar 

  16. Hartley, R. W. Trends biochem. Sci. 14, 450–454 (1989).

    Article  CAS  Google Scholar 

  17. McClure, B. A. et al. Nature 342, 955–957 (1989).

    Article  ADS  CAS  Google Scholar 

  18. De Block, M. et al. EMBO J. 6, 2513–2518 (1987).

    Article  CAS  Google Scholar 

  19. Deblaere, R. et al. Meth. Enzym. 153, 272–291 (1987).

    Google Scholar 

  20. Izant, J. G. & Weintraub, H. Science 229, 345–352 (1985).

    Article  ADS  CAS  Google Scholar 

  21. Cox, K. & Goldberg, R. in Plant Molecular Biology: A Practical Approach (ed. Shaw, C. H.) 1–35 (IRL, Oxford, 1988).

    Google Scholar 

  22. Jofuku, K. D. & Goldberg, R. B. in Plant Molecular Biology: A Practical Approach (ed. Shaw, C. H.) 37–66 (IRL, Oxford, 1988).

    Google Scholar 

  23. Maliga, P., Breznowitz, A. & Marton, A. Nature 244, 29–30 (1973).

    CAS  Google Scholar 

  24. Horsch, R. et al. Science 223, 496–498 (1984).

    Article  ADS  CAS  Google Scholar 

  25. Perez-Grau, L. & Goldberg, R. B. Pl. Cell 1, 1095–1109 (1989).

    Article  CAS  Google Scholar 

  26. Cornelissen, M. & Vandewiele, M. Nucleic Acids Res. 17, 19–29 (1989).

    Article  CAS  Google Scholar 

  27. De Block, M., De Brouwer, D. & Tenning, P. Pl. Physiol. 91, 695–701 (1989).

    Article  Google Scholar 

  28. Fromme, H. G., Pfantsch, M., Pfefferkorn, G. & Bystzieky, V. Microscopica Acta 73, 29–37 (1972).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mariani, C., Beuckeleer, M., Truettner, J. et al. Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 347, 737–741 (1990). https://doi.org/10.1038/347737a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/347737a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing