Abstract
Howard replies — Huxley points out that there is a discrepancy between recent mechanical recordings from single myosin molecules in vitro and earlier mechanical measurements from muscle fibres in vivo: the single-molecule recordings indicate a working distance and maximum duty ratio of 4-6 nm and 10-20%, respectively, only about half the values deduced from the fibrestudies. Although it is possible that technical limitations, for example misorientated heads, make the single-molecule recordings suspect, I argued in my ReviewArticle1 that the discrepancy might be only an apparent one. In particular, the smaller working distance does conform to many of the results from muscle, and the lower duty ratio can even provide a more satisfactory explanation for some of the experiments with muscle fibres. Huxley challenges this view by discussing three additional observations from fibres. But I believe that these too are mainly consistent with the smaller working stroke and duty ratio.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Howard, J. Nature 389, 561–567 (1997).
Finer, J. T., Simmons, R. M. & Spudich, J. A. Nature 368, 113–119 (1994).
Ford, L. E., Huxley, A. F. & Simmons, R. M. J. Physiol. 269, 441–515 (1977).
Huxley, A. F. & Tideswell, S. J. Musc. Res. Cell Motil. 17, 507–511 (1996).
Eisenberg, E., Hill, T. L. & Chen, Y. Biophys. J. 29, 195–227 (1980).
Ford, L. E., Huxley, A. F. & Simmons, R. M. J. Physiol. 361, 131–150 (1985).
Yanagida, T., Arata, T. & Oosawa, F. Nature 316, 366–369 (1985).
Higuchi, H. & Goldman, Y. E. Nature 352, 352–354 (1991).
Huxley, A. F. Prog. Biophys. Biophys. Chem. 7, 255–318 (1957).
Lovell, S. J., Knight, P. J. & Harrington, W. F. Nature 293, 664–666 (1981).
Yanagida, T., Arata, T. & Oosawa, F. Nature 352, 352–354 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Howard, J. How molecular motors work in muscle. Nature 391, 239–240 (1998). https://doi.org/10.1038/34569
Issue Date:
DOI: https://doi.org/10.1038/34569
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.