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Genetic diversity in Sargasso Sea bacterioplankton

Abstract

BACTERIOPLANKTON are recognized as important agents of biogeochemical change in marine ecosystems, yet relatively little is known about the species that make up these communities. Uncertainties about the genetic structure and diversity of natural bacterioplankton populations stem from the traditional difficulties associated with microbial cultivation techniques. Discrepancies between direct counts and plate counts are typically several orders of magnitude, raising doubts as to whether cultivated marine bacteria are actually representative of dominant planktonic species1–3. We have phylogenetically analysed clone libraries of eubacterial 16S ribosomal RNA genes amplified from natural populations of Sargasso Sea picoplankton by the polymerase chain reaction4. The analysis indicates the presence of a novel microbial group, the SAR 11 cluster, which appears to be a significant component of this oligotrophic bacterioplankton community. A second cluster of lineages related to the oxygenic phototrophs—cyanobacteria, prochlorophytes and chloroplasts—was also observed. However, none of the genes matched the small subunit rRNA sequences of cultivated marine cyanobacteria from similar habitats. The diversity of 16S rRNA genes observed within the clusters suggests that these bacterioplankton may be consortia of independent lineages sharing surprisingly distant common ancestors.

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References

  1. 1

    Jannasch, H. W. & Jones, G. E. Limnol. Oceanogr. 4, 128–139 (1959).

    ADS  Article  Google Scholar 

  2. 2

    Ferguson, R. L. & Rublee, P. Limnol. Oceanogr. 21, 141–145 (1976).

    ADS  Article  Google Scholar 

  3. 3

    Kogure, K., Simidu, U. & Taga, N. Can. J. Microbiol. 25, 415–420 (1979).

    CAS  Article  Google Scholar 

  4. 4

    Saiki, R. K. et al. Science 239, 487–491 (1988).

    ADS  CAS  Article  Google Scholar 

  5. 5

    Menzel, D. W. & Ryther, J. H. Deep-Sea Res. 6, 351–367 (1959).

    ADS  Google Scholar 

  6. 6

    Gutell, R. R. et al. Prog. Nucleic Acid Res. molec. Biol. 32, 155–216 (1985).

    CAS  Article  Google Scholar 

  7. 7

    Shuldiner, A., Nirula, A. & Roth, J. Nucleic Acids Res. 17, 4409 (1989).

    CAS  Article  Google Scholar 

  8. 8

    Giovannoni, S. J. et al. J. Bact. 170, 3584–3592 (1988).

    CAS  Article  Google Scholar 

  9. 9

    Waterbury, J. B., Watson, S. W., Valois, F. W. & Franks, D. G. Can. Bull. Fish. Aquat. Sci. 214, 71–120 (1987).

    Google Scholar 

  10. 10

    Waterbury, J. B., Willey, J. M., Franks, D. G., Valois, F. W. & Watson, S. W. Science 230, 74–76 (1985).

    ADS  CAS  Article  Google Scholar 

  11. 11

    Woese, C. R., Stackebrandt, E., Macke, T. J. & Fox, G. E. System. appl. Microbiol. 6, 143–152 (1985).

    CAS  Article  Google Scholar 

  12. 12

    Woese, C. Microbiol. Rev. 51, 221–271 (1987).

    CAS  PubMed  PubMed Central  Google Scholar 

  13. 13

    Giovannoni, S. J., DeLong, E., Olsen, G. J. & Pace, N. R. J. Bact. 170, 720–726 (1988).

    CAS  Article  Google Scholar 

  14. 14

    Sogin, M. L., Pechman, K. J., Zablen, L., Lewis, B. J. & Woese, C. R. J. Bact. 112, 13–22 (1972).

    CAS  PubMed  Google Scholar 

  15. 15

    Dunning, A. M., Talmud, P. & Humphries, S. E. Nucleic Acids Res. 16, 10393 (1988).

    CAS  Article  Google Scholar 

  16. 16

    Ho, S. et al. Gene 77, 51–59 (1989).

    CAS  Article  Google Scholar 

  17. 17

    Selander, R. K., Caugant, D. A. & Whittam, T. S. in Escherichia coli and Salmonella typhimurium, Cellular, Molecular Biology (eds Neidhardt, F. C. et al.) 1625–1648 (American Society for Microbiology, Washington, 1987).

    Google Scholar 

  18. 18

    McArthur, J. V., Kovacic, D. A. & Smith, M. H. Proc. natn. Acad. Sci. U.S.A. 85, 9621–9624 (1988).

    ADS  CAS  Article  Google Scholar 

  19. 19

    Proctor, L. M. & Fuhrman, J. A. Nature 343, 60–61 (1990).

    ADS  Article  Google Scholar 

  20. 20

    Wayne, L. G. et al. Int. J. system. Bact. 37, 463–464 (1987).

    Google Scholar 

  21. 21

    Olsen, G. J., Lane, D. J., Giovannoni, S. J., Pace, N. R. & Stahl, D. A. A. Rev. Microbiol. 40, 337–365 (1985).

    Article  Google Scholar 

  22. 22

    Sanger, F., Nicklen, S. & Coulsen, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    ADS  CAS  Article  Google Scholar 

  23. 23

    Jukes, T. H. & Cantor, C. R. in Mammalian Protein Metabolism (ed. Munro, H. N.) 21 (Academic, New York, 1969).

    Book  Google Scholar 

  24. 24

    Kimura, M. & Ohta, T. J. molec. Evol. 2, 87–90 (1972).

    ADS  CAS  Article  Google Scholar 

  25. 25

    Olsen, G. J. Meth. Enzym. 164, 793–812 (1988).

    CAS  Article  Google Scholar 

  26. 26

    Scharf, S. J., Horn, G. T. & Ehrlich, H. A. Science 233, 1076–1078 (1986).

    ADS  CAS  Article  Google Scholar 

  27. 27

    Brosius, J., Dull, T. J., Sleeter, D. D. & Noller, H. F. J. molec. Biol. 148, 107–127 (1981).

    CAS  Article  Google Scholar 

  28. 28

    Turner, S., Burger-Wiersma, T., Giovannoni, S. J., Mar, L. R. & Pace, N. R. Nature 337, 380–382 (1989).

    ADS  CAS  Article  Google Scholar 

  29. 29

    Stackebrandt, E. et al. Arch. Microbiol. 149, 547–556 (1988).

    CAS  Article  Google Scholar 

  30. 30

    Woese, C. R., Debrunner-Vossbrinck, B., Oyaizu, H., Stackebrandt, E. & Ludwig, W. Science 229, 762–765 (1985).

    ADS  CAS  Article  Google Scholar 

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Giovannoni, S., Britschgi, T., Moyer, C. et al. Genetic diversity in Sargasso Sea bacterioplankton. Nature 345, 60–63 (1990). https://doi.org/10.1038/345060a0

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