Abstract
ALL reactions involving reverse transcription of RNA are segre-gated from the cytosol within a subviral particle or capsid composed of the major capsid protein, the polymerase and the RNA tem-plate1. A key step in the formation of these particles is the selective encapsidation of the RNA template. Although an important gen-eral feature of the reverse transcription pathway, encapsidation has been carefully studied only for retrovirqses2–5. We have now examined the encapsidation reaction in a family of enveloped DNA viruses that replicate by reverse transcription—the hepatitis B viruses (hepadnaviruses). Our results indicate that the hepadnaviral polymerase (P) gene product is required for RNA packag-ing, and that the encapsidation function of the enzyme can be separated from its DNA polymerase activity. To our knowledge, this is the first description of a role for polymerase gene products in this step of the reverse transcription pathway.
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References
Fuetterer, J. & Hohn, T. Trends biochem. Sci. 12, 92–95 (1987).
Meric, C. & Spahr, P.-F. J. Virol. 60, 450–459 (1986).
Gorelick, R. J., Henderson, L. E., Hanser, J. P. & Rein, A. Proc. natn. Acad. Sci. U.S.A. 85, 8420–8424 (1988).
Mann, R., Mulligan, R. C. & Baltimore, D. Cell 33, 153–159 (1983).
Adam, M. A. & Miller, A. D. J. Virol. 62, 3802–3806 (1988).
Buscher, M., Reiser, W., Will, H. & Schaller, H. Cell 40, 717–724 (1985).
Enders, G. H., Ganem, D. & Varmus, H. E. J. Virol. 61, 35–41 (1987).
Chang, L.-J., Pryciak, P., Ganem, D. & Varmus, H. E. Nature 337, 364–367 (1989).
Schlicht, H.-J., Radziwill, G. & Schaller, H. Cell 56, 85–92 (1989).
Lavine, J., Hirsch, R. & Ganem, D. J. Virol. 63, 4257–4263 (1989).
Shields, A., Witte, O. N., Rothenberg, E. & Baltimore, D. Cell 14, 601–609 (1978).
Dickson, C., Eisenman, R., Fan, H., Hunter, E. & Teich, N. in RNA Tumor Viruses Ch. 6 (Cold Springer Harbor Laboratory, New York, 1982).
Eisenman, R. & Vogt, V. Biochem. biophys. Acta 473, 187–239 (1978).
Berg, D. in Mobile DNA 185–210 (American Society of Microbiology, New York, 1989).
Morisato, D., Way, J., Kim, H. & Kleckner, N. Cell 32, 799–807 (1983).
Bartenschlager, R. & Schaller, H. EMBO J. 7, 4185–4192 (1988).
Molnar-Kimber, K. L., Summers, J., Taylor, J. M. & Mason, W. S. J. Virol. 45, 165–172 (1983).
Prats, A. C. et al. EMBO J. 7, 1777–1783 (1988).
Hirsch, R., Colgrove, R. & Ganem, D. Virology 167, 136–142 (1988).
Sprengel, R., Kuhn, C., Will, H. & Schaller, H. J. Med. Virol. 15, 323–333 (1985).
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Hirsch, R., Lavine, J., Chang, Lj. et al. Polymerase gene products of hepatitis B viruses are required for genomic RNA packaging as well as for reverse transcription. Nature 344, 552–555 (1990). https://doi.org/10.1038/344552a0
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DOI: https://doi.org/10.1038/344552a0
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