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Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells

Nature volume 339, pages 446451 (08 June 1989) | Download Citation

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Abstract

Genes expressed in erythroid cells contain binding sites for a cell-specific factor believed to be an important regulator for this haematopoietic lineage. Using high-level transient expression in mammalian cells, we have identified complementary DNA encoding the murine protein. The factor, a new member of the zinc-finger family of DNA-binding proteins, is restricted to erythroid cells at the level of RNA expression and is closely homologous between mouse and man.

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Author information

Affiliations

  1. Division of Hematology-Oncology, Children's Hospital, Dana-Farber Cancer Institute, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA

    • Shih-Feng Tsai
    • , David I. K. Martin
    • , Leonard I. Zon
    • , Alan D. D'Andrea
    •  & Stuart H. Orkin
  2. Howard Hughes Medical Institute. Boston, Massachusetts 02115, USA

    • Shih-Feng Tsai
    • , David I. K. Martin
    • , Leonard I. Zon
    •  & Stuart H. Orkin
  3. Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA

    • Alan D. D'Andrea
  4. Genetics Institute, Cambridge, Massachusetts 02140, USA

    • Gordon G. Wong

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https://doi.org/10.1038/339446a0

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