Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells

Nature volume 339, pages446–451(1989)Cite this article

Abstract

Genes expressed in erythroid cells contain binding sites for a cell-specific factor believed to be an important regulator for this haematopoietic lineage. Using high-level transient expression in mammalian cells, we have identified complementary DNA encoding the murine protein. The factor, a new member of the zinc-finger family of DNA-binding proteins, is restricted to erythroid cells at the level of RNA expression and is closely homologous between mouse and man.

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Affiliations

  1. Division of Hematology-Oncology, Children's Hospital, Dana-Farber Cancer Institute, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Shih-Feng Tsai, David I. K. Martin, Leonard I. Zon, Alan D. D'Andrea & Stuart H. Orkin

  2. Howard Hughes Medical Institute, Boston, Massachusetts, 02115, USA

    Shih-Feng Tsai, David I. K. Martin, Leonard I. Zon & Stuart H. Orkin

  3. Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, 02142, USA

    Alan D. D'Andrea

  4. Genetics Institute, Cambridge, Massachusetts, 02140, USA

    Gordon G. Wong

Authors
  1. Shih-Feng Tsai
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  2. David I. K. Martin
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  3. Leonard I. Zon
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  4. Alan D. D'Andrea
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  5. Gordon G. Wong
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  6. Stuart H. Orkin
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Tsai, SF., Martin, D., Zon, L. et al. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells. Nature 339, 446–451 (1989). https://doi.org/10.1038/339446a0

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