Abstract
Antibodies against CD8 or CD4 antigens can prevent T-cell functions induced by T-cell targets1–3. As CD8 or CD4 antibodies can also initiate negative signals in T cells in the absence of appropriate targets4–6 it is not clear whether CD8 and CD4 molecules are directly involved in the interaction of T cells with their targets. In previous experiments we have introduced the T-cell receptor α-and β-chain genes from a CD8-positive cytolytic T cell specific for the antigen fluorescein (FL) and the H–2Dd molecule of the major histocompatibility complex (MHC) into a CD8-negative recipient cell. The CD8-positive donor cell lysed both FL-conjugated fibroblasts and lymphoblasts, which express relatively high and low amounts of H–2Dd molecules, respectively. In contrast the CDS-negative transfectant lysed FL-conjugated fibroblasts only7. Here we show that recognition of FL-conjugated lymphoblasts by the transfectant is enhanced by supertransfecting it with the CD8 gene.
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References
MacDonald, H. R., Glasebrook, A. L., Bron, C., Kelsoe, A. & Cerrotini, J. C. Immun. Rev. 68, 89–115 (1982).
Marrack, P. et al. J. exp. Med. 158, 1077–1091 (1983).
Greenstein, J. L., Malissen, B. & Burakoff, S. J. J. exp. Med. 162, 369–374 (1985).
Bank, I. & Chess, L. J. exp. Med. 162, 1294–1303 (1985).
Fleischer, B., Schrezenmeier, H. & Wagner, H. J. Immun. 136, 1625–1628 (1986).
Fleischer, B., Schendel, D. J. & von Steldern, D. Eur. J. Immun. 16, 741–746 (1986).
Dembić, Z. et al. Nature 320, 232–238 (1986).
Ledbetter, J. A. & Herzenberg, L. A. Immun. Rev. 47, 63–90 (1979).
Bernard, H. V., Krämmer, G. & Röwekamp, W. G. Expl Cell Res. 158, 237–243 (1985).
Folger, K. R., Wong, E. A., Wahl, G. & Capecchi, M. R. Molec. cell. Biol. 2, 1372–1387 (1982).
Liew, C. W., Zamoyska, R. & Pames, J. R. J. Immun. 137, 1037–1043 (1986).
Haas, W., Pohlit, H. & von Boehmer, H. Eur. J. Immun. 9, 868–874 (1979).
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Dembić, Z., Haas, W., Zamoyska, R. et al. Transfection of the CD8 gene enhances T-cell recognition. Nature 326, 510–511 (1987). https://doi.org/10.1038/326510a0
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DOI: https://doi.org/10.1038/326510a0
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