Abstract
Expression of the middle-T antigen of polyomavirus is sufficient to induce transformation of fibroblasts in culture and tumour formation in whole animals1–4. Middle-T can form a complex with the cellular src gene product (p60c-src) and can be phosphorylated by p60c-src in vitro5,6. Studies using middle-T mutants have suggested that the association of middle-T with pc-src may be necessary but not sufficient for transformation7–9. Therefore, we addressed the possibility that middle-T could interact with other tyrosine protein kinases structurally related to p60c-src. Using antibody raised against a fusion protein between β-galactosidase and amino-terminal sequences of p90gag-yes from Y73 virus (anti-yes antibody)10, we have found that middle-T can associate with and be phosphorylated by the c-yes proto-oncogene product, a protein of relative molecular mass (Mr) 62,000 (62K). This raises the possibility that the middle-T–c-yes complex contributes to transformation by polyomavirus.
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Kornbluth, S., Sudol, M. & Hanafusa, H. Association of the polyomavirus middle-T antigen with c-yes protein. Nature 325, 171–173 (1987). https://doi.org/10.1038/325171a0
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DOI: https://doi.org/10.1038/325171a0
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