Abstract
Cloning and sequence analysis of DNA complementary to porcine cerebral messenger RNA encoding the muscarinic acetylcholine receptor predict the complete amino-acid sequence of this protein. Expression of the complementary DNA produces functional muscarinic receptor in Xenopus oocytes. The muscarinic receptor is homologous with the β-adrenergic receptor and rhodopsin in both amino-acid sequence and suggested transmembrane topography.
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References
Burgen, A. S. V. Trends pharmac. Sci. Suppl. 5, 1–3 (1984).
Brown, D. Nature 319, 358–359 (1986).
Gilman, A. G. Cell 36, 577–579 (1984).
Hammer, R., Berrie, C. P., Birdsall, N. J. M., Burgen, A. S. V. & Hulme, E. C. Nature 283, 90–92 (1980).
Birdsall, N. J. M. & Hulme, E. C. Trends pharmac. Sci. 4, 459–463 (1983).
Watson, M. et al. Trends pharmac. Sci. Suppl. 5, 46–55 (1986).
Haga, K. & Haga, T. J. biol. Chem. 260, 7927–7935 (1985).
Haga, K. et al. Nature 316, 731–733 (1985).
Haga, K., Haga, T. & Ichiyama, A. J. biol. Chem. 261, 10133–10140 (1986).
Hewick, R. M., Hunkapiller, M. W., Hood, L. E. & Dreyer, W. J. J. biol. Chem. 256, 7990–7997 (1981).
Okayama, H. & Berg, P. Molec. cell Biol. 2, 161–170 (1982).
Grantham, R., Gautier, C., Gouy, M., Jacobzone, M. & Mercier, R. Nucleic Acids Res. 9, r43–r74 (1981).
Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
Kozak, M. Nucleic Acids Res. 12, 857–872 (1984).
Shirakawa, O., Kuno, T. & Tanaka, C. Biochem. biophys. Res. Commun. 115, 814–819 (1983).
Grefrath, S. P. & Reynolds, J. A. Proc. natn. Acad. Sci. U.S.A. 71, 3913–3916 (1974).
Peterson, G. L., Rosenbaum, L. C., Broderick, D. J. & Schimerlik, M. I. Biochemistry 25, 3189–3202 (1986).
Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).
Dixon, R. A. F. et al. Nature 321, 75–79 (1986).
Green, S. et al. Nature 320, 134–139 (1986).
Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).
Engelman, D. M., Goldman, A. & Steitz, T. A. Meth. Enzym. 88, 81–88 (1982).
Ovchinnikov, Y. A. FEBS Lett. 148, 179–191 (1982).
Hargrave, P. A. et al. Biophys. Struct. Mech. 9, 235–244 (1983).
Nathans, J. & Hogness, D. S. Cell 34, 807–814 (1983).
Toh, H., Hayashida, H. & Miyata, T. Nature 305, 827–829 (1983).
Dayhoff, M. O., Schwartz, R. M. & Orcutt, B. C. in Atlas of Protein Sequence and Structure Vol. 5 Suppl. 3 (ed. Dayhofi, M. O.) 345–352 (National Biomedical Research Foundation, Silver Spring, Maryland, 1978).
Henderson, R. & Unwin, P. N. T. Nature 257, 28–32 (1975).
Khorana, H. G. et al. Proc. natn. Acad. Sci. U.S.A. 76, 5046–5050 (1979).
Ovchinnikov, Y. A., Abdulaev, N. G., Feigina, M. Y., Kiselev, A. V. & Lobanov, N. A. FEBS Lett. 100, 219–224 (1979).
Hargrave, P. A. Prog. Retinal Res. 1, 1–51 (1982).
Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 852–862 (1975).
Hubbard, S. C. & Ivatt, R. J. A. Rev. Biochem. 50, 555–583 (1981).
Benovic, J. L., Mayor, F. Jr, Somers, R. L., Caron, M. G. & Lefkowitz, R. J. 321, 869–872 (1986).
Krebs, E. G. & Beavo, J. A. A. Rev. Biochem. 48, 923–959 (1979).
Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).
Kusano, K., Miledi, R. & Stinnakre, J. J. Physiol., Lond. 328, 143–170 (1982).
Dascal, N., Landau, E. M. & Lass, Y. J. Physiol., Lond. 352, 551–574 (1984).
Sugiyama, H., Hisanaga, Y. & Hirono, C. Brain Res. 338, 346–350 (1985).
Mishina, M. et al. Nature 307, 604–608 (1984).
Barnard, E. A., Miledi, R. & Sumikawa, K. Proc. R. Soc. B215, 241–246 (1982).
Barish, M. E. J. Physiol., Lond. 342, 309–325 (1983).
Yamamura, H. I. & Snyder, S. H. Proc. natn. Acad. Sci. U.S.A. 71, 1725–1729 (1974).
Hulme, E. C., Birdsall, N. J. M., Burgen, A. S. V. & Mehta, P. Molec. Pharmac. 14, 737–750 (1978).
Berrie, C. P. et al. Trends pharmac. Sci. Suppl. 7, 8–13 (1986).
Harden, T. K. et al. Trends pharmac. Sci. Suppl. 7, 14–18 (1986).
Fung, B. K.-K., Hurley, J. B. & Stryer, L. Proc. natn. Acad. Sci. U.S.A. 78, 152–156 (1981).
Kanaho, Y. et al. J. biol. Chem. 259, 7378–7381 (1984).
Nathans, J., Thomas, D. & Hogness, D. S. Science 232, 193–202 (1986).
Nakayama, N., Miyajima, A. & Arai, K. EMBO J. 4, 2643–2648 (1985).
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).
Boyer, H. W. & Roulland-Dussoix, D. J. molec. Biol. 41, 459–472 (1969).
Wahl, G. M., Padgett, R. A. & Stark, G. R. J. biol. Chem. 254, 8679–8689 (1979).
Hanahan, D. & Meselson, M. Gene 10, 63–67 (1980).
Ito, H., Ike, Y., Ikuta, S. & Itakura, K. Nucleic Acids Res. 10, 1755–1769 (1982).
Konarska, M. M., Padgett, R. A. & Sharp, P. A. Cell 38, 731–736 (1984).
Gurdon, J. B. The Control of Gene Expression in Animal Development (Clarendon, Oxford, 1974).
Sakmann, B. et al. Nature 318, 538–543 (1985).
Nukada, T., Haga, T. & Ichiyama, A. Molec. Pharmac. 24, 366–373 (1983).
McMaster, G. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 74, 4835–4838 (1977).
Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).
Mishina, M. et al. Nature 321 406–411 (1986).
Noller, H. F. A. Rev. Biochem. 53, 119–162 (1984).
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Kubo, T., Fukuda, K., Mikami, A. et al. Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor. Nature 323, 411–416 (1986). https://doi.org/10.1038/323411a0
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DOI: https://doi.org/10.1038/323411a0
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