Abstract
Transcription of eukaryotic transfer RNA genes involves, as a primary event, the stable binding of a protein factor to the intragenic promoter1–4. The internal control region is composed of two non-contiguous conserved sequence elements, the A and B blocks. These are variably spaced depending on the genes5–8. τ, a large transcription factor purified from yeast cells4, interacts with these two control elements as shown by DNase I footprinting, exonuclease digestion, dimethyl sulphate protection experiments9,10 and by analysis of point mutations. Here we used a limited proteolysis treatment to obtain a smaller form of τ with drastically altered DNA binding properties. A protease-resistant domain interacts solely with the B block region of tRNA genes.
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References
Klemenz, R., Stillman, D. J. & Geiduschek, E. P. Proc. natn. Acad. Sci. U.S.A. 79, 6191–6195 (1982).
Lassar, A. B., Martin, P. L. & Roeder, R. G. Science 222, 740–748 (1983).
Schaack, J., Sharp, S., Dingermann, T. & Söll, D. J. biol. Chem. 258, 2447–2453 (1983).
Ruet, A., Camier, S., Smagowicz, W., Sentenac, A. & Fromageot, P. EMBO J. 3, 343–350 (1984).
Galli, G., Hofstetter, H. & Birnstiel, M. L. Nature 294, 626–631 (1981).
Ciliberto, G., Castagnoli, L., Melton, D. A. & Cortese, R. Curr. Topics dev. Biol. 18, 59–88 (1983).
Allison, D. S., Goh, S. H. & Hall, B. D. Cell 34, 655–664 (1983).
Dingermann, T., Sharp, S., Schaack, J. & Söll, D. J. biol. Chem. 258, 10395–10402 (1983).
Stillman, D. J., Caspers, P. & Geiduschek, E. P. Cell 40, 311–317 (1985).
Camier, S., Gabrielsen, O., Baker, R. & Sentenac, A. EMBO J. 4, 491–500 (1985).
Baker, R. E., Gabrielsen, O. & Hall, B. D. J. biol. Chem. 261, 5275–5282 (1986).
Huet, J. et al. EMBO J. 4, 3539–3547 (1985).
Stillman, D. J., Sivertsen, A. L., Zentner, P. G. & Geiduschek, E. P. J. biol. Chem. 259, 7955–7962 (1984).
Stillman, D. L., Better, M. & Geiduschek, E. P. J. molec. Biol. 185, 451–455 (1985).
Smith, D. R., Jackson, I. J. & Brown, D. D. Cell 37, 645–652 (1984).
Miller, J., McLachlan, A. D. & Klug, A. EMBO J. 4, 1609–1614 (1985).
Rosenberg, U. B. et al. Nature 319, 336–339 (1986).
Hartshorne, T. A., Blumberg, H. & Young, E. T. Nature 320, 283–287 (1986).
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Marzouki, N., Camier, S., Ruet, A. et al. Selective proteolysis defines two DNA binding domains in yeast transcription factor τ. Nature 323, 176–178 (1986). https://doi.org/10.1038/323176a0
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DOI: https://doi.org/10.1038/323176a0
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