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Fluorescence detection in automated DNA sequence analysis

Naturevolume 321pages674679 (1986) | Download Citation

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Abstract

We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer.

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Author information

Author notes

  1. Charles R. Connell and Cheryl Heiner: Applied Biosystems, Inc., Foster City, California 94404, USA

Affiliations

  1. Division of Biology, California Institute of Technology, Pasadena, California, 91125, USA

    • Lloyd M. Smith
    • , Jane Z. Sanders
    • , Robert J. Kaiser
    • , Peter Hughes
    • , Chris Dodd
    • , Charles R. Connell
    • , Cheryl Heiner
    • , Stephen B. H. Kent
    •  & Leroy E. Hood

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https://doi.org/10.1038/321674a0

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