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A molecular timescale for vertebrate evolution

Nature volume 392, pages 917920 (30 April 1998) | Download Citation

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Abstract

A timescale is necessary for estimating rates of molecular and morphological change in organisms and for interpreting patterns of macroevolution and biogeography1,2,3,4,5,6,7,8,9. Traditionally, these times have been obtained from the fossil record, where the earliest representatives of two lineages establish a minimum time of divergence of these lineages10. The clock-like accumulation of sequence differences in some genes provides an alternative method11 by which the mean divergence time can be estimated. Estimates from single genes may have large statistical errors, but multiple genes can be studied to obtain a more reliable estimate of divergence time1,12,13. However, until recently, the number of genes available for estimation of divergence time has been limited. Here we present divergence-time estimates for mammalian orders and major lineages of vertebrates, from an analysis of 658 nuclear genes. The molecular times agree with most early (Palaeozoic) and late (Cenozoic) fossil-based times, but indicate major gaps in the Mesozoic fossil record. At least five lineages of placental mammals arose more than 100 million years ago, and most of the modern orders seem to have diversified before the Cretaceous/Tertiary extinction of the dinosaurs.

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References

  1. 1.

    , & Biochemical evolution. Annu. Rev. Biochem. 46, 573–639 (1977).

  2. 2.

    Molecular Evolutionary Genetics (Columbia Univ. Press, New York, 1987).

  3. 3.

    Mammalian phylogeny: shaking the tree. Nature 356, 121–125 (1992).

  4. 4.

    Primate origins: plugging the gaps. Nature 363, 223–234 (1993).

  5. 5.

    Molecular Markers, Natural History and Evolution (Chapman & Hall, New York, 1994).

  6. 6.

    An Outline of Phanerozoic Biogeography (Oxford Univ. Press, New York, 1994).

  7. 7.

    , & The Mammalian Molecular Clock (R. G. Landes, Austin, TX, 1995).

  8. 8.

    & Cells, Embryos, and Evolution (Blackwell Scientific, Malden, Massachusetts, 1997).

  9. 9.

    Before the Backbone (Chapman & Hall, New York, NY, 1996).

  10. 10.

    The Fossil Record 2 (Chapman & Hall, London, 1993).

  11. 11.

    On the molecular evolutionary clock. J. Mol. Evol. 26, 34–46 (1987).

  12. 12.

    , , & Continental breakup and the ordinal diversification of birds and mammals. Nature 381, 226–229 (1996).

  13. 13.

    , & Phylogenetic test of the molecular clock and linearized tree. Mol. Biol. Evol. 12, 823–833 (1995).

  14. 14.

    Vertebrate Paleontology (Chapman & Hall, New York, 1997).

  15. 15.

    Fossil evidence for a late Cretaceous origin of “hoofed” mammals. Science 272, 1150–1153 (1996).

  16. 16.

    Interrelationships of Mesozoic mammals. Hist. Biol. 6, 185–202 (1992).

  17. 17.

    & Mass survival of birds across the Cretaceous-Tertiary boundary: molecular evidence. Science 275, 1109–1113 (1997).

  18. 18.

    , & Anthropoid origins. Science 275, 797–804 (1997).

  19. 19.

    in Function, Phylogeny, and Fossils (eds Begun, D. R., Ward, C. V. & Rose, M. D.) 269–290 (Plenum, New York, 1997).

  20. 20.

    Genetic and morphological records of the Hominoidea and hominid origins: a synthesis. Mol. Phyl. Evol. 5, 155–168 (1996).

  21. 21.

    & Earliest known Old World monkey skull. Nature 388, 368–371 (1997).

  22. 22.

    , & The age of the Mus-Rattus divergence: paleontological data compared with the molecular clock. C. R. Acad. Sci. Paris 302, 917–922 (1986).

  23. 23.

    An Improved Estimate of the Mouse-Rat Divergence Time and Rates of Amino Acid Substitution in Mammals and BirdsThesis, Pennsylvania State Univ.((1996).

  24. 24.

    Vertebrate Paleontology and Evolution (W. H. Freeman and Co., New York, 1988).

  25. 25.

    et al. Endemic African mammals shake the phylogenetic tree. Nature 388, 61–63 (1997).

  26. 26.

    , & HOVERGEN: a database of homologous vertebrate genes. Nucleic Acids Res. 22, 2360–2365 (1994).

  27. 27.

    Simple methods for testing the molecular evolutionary clock hypothesis. Genetics 135, 599–607 (1993).

  28. 28.

    , & MEGA: Molecular Evolutionary Genetic Analysis (Pennsylvania State Univ., 1993).

  29. 29.

    , & APaleocene proboscidean from Morocco. Nature 383, 68–70 (1996).

  30. 30.

    Phylogeny of the major tetrapod groups: morphological data and divergence dates. J. Mol. Evol. 30, 409–424 (1990).

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Acknowledgements

We thank L. Poling, A. Beausang, and R. Padmanabhan for assistance with sequence data retrieval; A. Beausang for artwork; A. G. Clark, C. A. Hass, I. Jakobsen, M. Nei, C. R. Rao, and A.Walker for comments and discussion; and L. Duret for instructions on use of the HOVERGEN database. This work was supported in part by grants to M. Nei (NIH and NSF) and S.B.H. (NSF).

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  1. Department of Biology and Institute of Molecular Evolutionary Genetics, 208 Mueller Laboratory, Pennsylvania State University, University Park, Pennsylvania 16802, USA

    • Sudhir Kumar
    •  & S. Blair Hedges

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Correspondence to S. Blair Hedges.

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https://doi.org/10.1038/31927

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