Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Repression of simian virus 40 early transcription by viral DNA replication in human 293 cells

Nature volume 317, pages 172–175 (1985)Cite this article

Abstract

The small DNA tumour virus simian virus 40 (SV40) has served as an excellent model for many studies on the mechanism and control of gene expression in eukaryotic cells. The SV40 early region produces two protein products. One product (large-T antigen) is known both to repress early viral transcription1,2 and to stimulate viral replication3,4 by binding to specific sites in the origin–promoter region5–7. The early promoter has several similarities to other RNA polymerase II promoters, for example, it possesses a TATA box, an upstream element8–10 and an enhancer11,12. However, the SV40 early promoter differs from other known RNA polymerase II promoters in that the origin of viral DNA replication is embedded within it13,14. Here we show that the SV40 early region is expressed at an extremely low level following its introduction ito human 293 cells, contrasting with results observed in a large number of other cell lines15. We show further that the lack of expression is due to repression of transcription from the SV40 early promoter by viral DNA replication which occurs efficiently in 293 cells.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Tegtmeyer, P., Schwarte, M., Collins, J. K. & Rundell, K. J. Virol. 16, 168–178 (1975).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Reed, S. I., Stark, G. R. & Alwine, J. C. Proc. natn. Acad. Sci. U.S.A. 73, 3083–3087 (1976).

    Article  ADS  CAS  Google Scholar 

  3. Tegtmeyer, P. J. Virol. 10, 591 (1972).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Chou, J. Y., Avila, J. & Martin, R. G. J. Virol. 14, 116–124 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Hansen, U., Tenen, D. G., Livingstone, D. M. & Sharp, P. A. Cell 27, 603–612 (1981).

    Article  CAS  PubMed  Google Scholar 

  6. Rio, D., Robbins, A., Myers, R. & Tjian, R. Proc. natn. Acad. Sci. U.S.A. 77, 5706–5710 (1980).

    Article  ADS  CAS  Google Scholar 

  7. Prives, C., Covey, L., Scheller, A. & Gluzman, Y. Molec. cell. Biol. 3, 1958–1966 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Manley, J. L. Prog. Nucleic Acids Res. molec. Biol. 30, 195–244 (1983).

    Article  CAS  Google Scholar 

  9. Benoist, C. & Chambon, P. Nature 290, 304–309 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Everett, R. D., Baty, D. & Chambon, P. Nucleic Acids Res. 11, 2447–2464 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Gruss, P., Dhar, R. & Khoury, G. Proc. natn. Acad. Sci. U.S.A 78, 943–947 (1981).

    Article  ADS  CAS  Google Scholar 

  12. Moreau, P. et al. Nucleic Acids Res. 9, 6047–6068 (1981).

    Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

  13. Danna, K. S. & Nathans, D. Proc. natn. Acad. Sci. U.S.A. 69, 3097–4001 (1972).

    Article  ADS  CAS  Google Scholar 

  14. Bergsma, D. J., Olive, D. M., Hartzell, S. W. & Subramanian, K. N. Proc. natn. Acad. Sci. U.S.A. 79, 381–385 (1982).

    Article  ADS  CAS  Google Scholar 

  15. Khoury, G., Byrne, J. C., Takemoto, K. K. & Martin, M. A. J. Virol. 11, 54–60 (1973).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Graham, F. L., Smiley, J., Russell, W. C. & Nairn, R. J. gen. Virol. 36, 59–72 (1977).

    Article  CAS  PubMed  Google Scholar 

  17. Aiello, L., Guilfoyle, R., Huebner, K. & Weinmann, R. Virology 94, 460–469 (1979).

    Article  CAS  PubMed  Google Scholar 

  18. Jones, N. & Shenk, T. Proc. natn. Acad. Sci. U.S.A. 76, 3665–3669 (1979).

    Article  ADS  CAS  Google Scholar 

  19. Berk, A. J., Lee, F., Harrison, T., Williams, J. & Sharp, P. A. Cell 17, 937–944 (1979).

    Article  Google Scholar 

  20. Green, M. R., Treisman, R. & Maniatis, T. Cell 35, 137–148 (1983).

    Article  CAS  PubMed  Google Scholar 

  21. Schrier, P. I., Bernards, R., Vaessen, R. J., Houweling, A. & van der Eb, A. J. Nature 305, 771–775 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  22. Wigler, M. et al. Proc. natn. Acad. Sci. U.S.A. 76, 1373–1376 (1979).

    Article  ADS  CAS  Google Scholar 

  23. Verderame, M., Alcorta, D., Egnor, M., Smith, K. & Pollack, R. Proc. natn. Acad. Sci. U.S.A. 77, 6624–6628 (1980).

    Article  ADS  CAS  Google Scholar 

  24. Lewis, E. D. et al. Proc. natn. Acad. Sci. U.S.A. 80, 7065–7069 (1983).

    Article  ADS  CAS  Google Scholar 

  25. Chen, S. et al. J. Virol. 48, 492–502 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  26. Gluzman, Y., Sambrook, J. F. & Frisque, R. J. Proc. natn. Acad. Sci U.S.A. 77, 3898–3902 (1980).

    Article  ADS  CAS  Google Scholar 

  27. DiMaio, D. & Nathans, D. J. molec. Biol. 156, 531–548 (1982).

    Article  CAS  PubMed  Google Scholar 

  28. Gluzman, Y., Davison, J., Oren, M. & Winocour, E. J. Virol. 22, 256–266 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  29. Lebkowski, J. S., Clancy, S. & Calos, M. P. Nature 317, 169–171 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  30. Hirt, B. J. molec. Biol. 26, 365–369 (1967).

    Article  CAS  PubMed  Google Scholar 

  31. Southern, E. M. J. molec. BioL 98, 503–517 (1975).

    Article  CAS  PubMed  Google Scholar 

  32. Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol 113, 237–252 (1977).

    Article  CAS  PubMed  Google Scholar 

  33. Shaw, A. R. & Ziff, E. B. Cell 22, 905–916 (1980).

    Article  CAS  PubMed  Google Scholar 

  34. Akusjarvi, G. & Persson, H. Nature 292, 420–426 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  35. Thomas, G. P. & Mathews, M. B. Cell 22, 523–533 (1980).

    Article  CAS  PubMed  Google Scholar 

  36. Treisman, R., Green, M. R. & Maniatis, T. Proc. natn, Acad. Sci U.S.A. 80, 7428–7432 (1983).

    Article  ADS  CAS  Google Scholar 

  37. Miller, A. M. & Nasmyth, K. A. Nature 312, 247–251 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  38. Chow, K.-C. & Pearson, G. D. Proc natn. Acad. Sci. U.S.A. 82, 2247–2251 (1985).

    Article  ADS  CAS  Google Scholar 

  39. Frost, E. & Williams, J. J. Virol 91, 39–50 (1978).

    Article  CAS  Google Scholar 

  40. Kimura, G. & Dulbecco, R. Virology 49, 394–403 (1972).

    Article  CAS  PubMed  Google Scholar 

  41. Harlow, E., Crawford, L. V., Pim, D. C. & Williamson, N. M. J. Virol 39, 861–869 (1981).

    CAS  PubMed  PubMed Central  Google Scholar 

  42. Lewis, E. D., Fu, X.-Y. & Manley, J. L. ICN-UCLA Symp. molec. Biol. Dev. 19, 351–360 (1984).

    CAS  Google Scholar 

  43. Buchman, G., Burnett, L. & Berg, P. in DNA Tumor Viruses 2nd edn (ed. Tooze, J.) 799–841 (Cold Spring Harbor Laboratory, New York, 1981).

    Google Scholar 

  44. Tooze, J. (ed.) DNA Tumor Viruses 2nd edn (Cold Spring Harbor Laboratory, New York, 1981).

  45. Gluzman, Y. Cell 23, 175–182 (1981).

    Article  CAS  PubMed  Google Scholar 

  46. Berk, A. J. & Sharp, P. A. Proc. natn. Acad. Sci. U.S.A. 75, 1274–1278 (1978).

    Article  ADS  CAS  Google Scholar 

  47. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–561 (1980).

    Article  CAS  PubMed  Google Scholar 

  48. Southern, E. M. J. molec. BioL 98, 503–517 (1975).

    Article  CAS  PubMed  Google Scholar 

  49. Manos, M. M. & Gluzman, Y. Molec. cell. Biol. 4, 1125–1133 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. Lewis, E. D. and Manley, J. L. Molec. cell Biol. (in the press).

Download references

Author information

Authors and Affiliations

  1. Department of of Biological Sciences, Columbia University, New York, New York, 10027, USA

    E. Diann Lewis & James L. Manley

Authors
  1. E. Diann Lewis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. James L. Manley
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lewis, E., Manley, J. Repression of simian virus 40 early transcription by viral DNA replication in human 293 cells. Nature 317, 172–175 (1985). https://doi.org/10.1038/317172a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/317172a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing