Abstract
The human transcription factor Sp1 binds upstream of certain viral and cellular promoters and activates initiation of RNA synthesis from these promoters by RNA polymerase II. The Sp1 binding sites of both simian virus 40 and a related monkey promoter contain multiple copies of the sequence GGGCGG, three or four of these sequences forming close contacts with Sp1. The clustered contacts fall on one strand of the DNA and are arranged similarly in the major groove of the DNA helix in both promoters.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Dynan, W. S. & Tjian, R. Cell 32, 669–680 (1983).
Dynan, W. S. & Tjian, R. Cell 35, 79–87 (1983).
Parker, C. S. & Topol, J. Cell 37, 273–283 (1984).
Myers, R. M., Rio, D. C., Robbins, A. K. & Tjian, R. Cell 25, 373–384 (1981).
Fromm, M., Berg, P. & J. molec. appl. Genet. 1, 457–481 (1982).
Lebowitz, P. & Ghosh, P. K. J. Virol. 41, 449–461 (1982).
Everett, R. D., Batty, D. & Chambon, P. Nucleic Acids Res. 11, 2447–2464 (1983).
Hartzell, S. W., Yamaguchi, J. & Subramanian, K. N. Nucleic Acids Res. 11, 1601–1616 (1983).
Rio, D. & Tjian, R. J. molec. appl Genet. 2, 423–435 (1984).
Hansen, U. & Sharp, P. A. EMBO J. 2, 2293–2303 (1983).
McCutchan, T. F. & Singer, M. F. Proc. natn. Acad. Sci. U.S.A. 78, 95–99 (1981).
Queen, C., Lord, S. T., McCutchan, T. F. & Singer, M. F. Molec. cell. Biol. 1, 1061–1068 (1981).
Saffer, J. D. & Singer, M. F. Nucleic Adas Res. 12, 4769–4788 (1984).
Lawley, P. D. & Brookes, P. Biochem. J. 89, 127–128 (1963).
Gilbert, W., Maxam, A. M. & Mirzabekov, A. in Control of Ribosome Synthesis (eds Kjeldgaard, N. C. & Maaloe, O.) 139–143 (Munksgard, Copenhagen, 1976).
Tooze, J. Molecular Biology of Tumor Viruses 2nd edn, Pt. 2 (Cold Spring Harbor Laboratory, New York, 1981).
Pabo, C. O. & Sauer, R. T. A. Rev. Biochem. 53, 291–321 (1984).
Sakonju, S. & Brown, D. D. Cell 31, 395–405 (1982).
Jongstra, J. et al. Nature 307, 708–714 (1984).
Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–580 (1980).
Galas, D. & Schmitz, A. Nucleic Acids Res. 5, 3157–3170 (1978).
Jones, K. A. & Tjian, R. Cell 36, 155–162 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gidoni, D., Dynan, W. & Tjian, R. Multiple specific contacts between a mammalian transcription factor and its cognate promoters. Nature 312, 409–413 (1984). https://doi.org/10.1038/312409a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/312409a0
This article is cited by
-
Tolfenamic acid decreases c-Met expression through Sp proteins degradation and inhibits lung cancer cells growth and tumor formation in orthotopic mice
Investigational New Drugs (2011)
-
Curcumin decreases the expression of Pokemon by suppressing the binding activity of the Sp1 protein in human lung cancer cells
Molecular Biology Reports (2010)
-
Molecular characterization of the porcine testis-specificphosphoglycerate kinase 2 (PGK2) gene and its association with male fertility
Mammalian Genome (2004)
-
Analysis of the genetic information of a DNA segment of a new virus from silkworm
Archives of Virology (1995)
-
Inverse PCR-mediated cloning of the promoter for the rat vasopressin V2 receptor gene
Pfl�gers Archiv European Journal of Physiology (1995)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
