Abstract
Immunoglobulin genes are normally expressed only in cells of the B lymphocyte lineage after a variable (V) and constant (C) gene rearrangement has occurred1. To study the control of immunoglobulin gene expression in a defined situation, we have produced transgenic mice by microinjecting a rearranged mouse immunoglobulin k gene (designated pB1-14) into fertilized mouse eggs2. We present here the analysis of six different k-transgenic mouse lines. All the transgenic mice express the microinjected k gene in a completely tissue-specific fashion. Transcripts from pB1-14 are found at a high level in the spleen, but are undetectable in nonlymphoid tissues of testis, liver, kidney, heart, muscle, brain and thyroid gland. In lymphoid cell subpopulations, the level of pB1-14 transcripts is correlated with the relative number of B cells; there is no correlation with the proportion of T lymphocytes. We concluded, therefore, that the microinjected k gene contains target sequences for B lymphocyte-specific gene activation signals that override the influence of the integration site.
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References
Hozumi, N. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 73, 3628–3632 (1976).
Brinster, R. L. et al. Nature 306, 332–336 (1983).
Walfield, A. M., Storb, U., Selsing, E. & Zentgraf, H. Nucleic Acids Res. 20, 4689–4707 (1980).
Thomas, P. S. Proc. natn. Acad. sci. U.S.A. 77, 5201–5205 (1980).
Rokhlin, O. V., Petrosyan, M. N., Ibraghimov, A. R., Vengerova, T. I. & Bogachova, G. T. Eur. J. Immun. 13, 397–403 (1983).
Kemp, D. J., Wilson, A., Harris, A. W. & Shortman, K. Nature 286, 168–170 (1980).
Cooper, M. D., Kearny, J. & Scher, I. in Fundamental Immunology (ed. Paul, W. E.) 43–55 (Raven, New York, 1984).
Butcher, E. C. & Weissman, I. L. in Fundamental Immunology (ed. Paul, W. E.) 109–127 (Raven, New York, 1984).
Lacy, E., Roberts, S., Evans, E., Burtenshaw, M. D. & Constantini, F. D. Cell 34, 343–358 (1983).
Flavell, A. Nature 305, 96–97 (1983).
Chung, S.-Y., Folsom, V. & Wooley, J. Proc. natn. Acad. Sci. U.S.A. 80, 2427–2431 (1983).
Emorine, L., Kuehl, M., Weir, L., Leder, P. & Max, E. E. Nature 304, 447–449 (1983).
Queen, C. & Baltimore, D. Cell 33, 741–748 (1983).
Picard, D. & Schaffner, W. Nature 307, 80–82 (1984).
Wysocki, L. J. & Sato, V. L. Proc. natn. Acad. Sci. U.S.A. 75, 2844–2848 (1978).
Henry, C. in Selected Methods in Cellular Immunology (eds Mishell, B. B. & Shiigi, S. M.) 182–185 (W. H. Freeman, San Francisco, 1980).
McMaster, G. K. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 74, 4835–4838 (1977).
Denhardt, D. T. Biochem. biophys. Res. Commun. 23, 641–652 (1966).
Walfield, A., Selsing, E., Arp, B. & Storb, U. Nucleic Acids Res. 9, 1101–1109 (1981).
Messing, G., Crea, R. & Seeburg, P. H. Nucleic Acids Res. 9, 309–321 (1981).
Hamlyn, P. H., Gait, M. J. & Milstein, C. Nucleic Acids Res. 9, 4485–4494 (1981).
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Storb, U., O'Brien, R., McMullen, M. et al. High expression of cloned immunoglobulin k gene in transgenic mice is restricted to B lymphocytes. Nature 310, 238–241 (1984). https://doi.org/10.1038/310238a0
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DOI: https://doi.org/10.1038/310238a0
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