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Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector

Abstract

Foreign genes introduced into plant cells with Ti-plasmid vectors are not expressed. We have constructed an expression vector derived from the promoter sequence of nopaline synthase, and have inserted the coding sequences of the octopine synthase gene and a chloramphenicol acetyltransferase gene into this vector. These chimaeric genes are functionally expressed in plant cells after their transfer via a Ti-plasmid of Agrobacterium tumefaciens.

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Herrera-Estrella, L., Depicker, A., Van Montagu, M. et al. Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector. Nature 303, 209–213 (1983). https://doi.org/10.1038/303209a0

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