Abstract
Several studies have shown that short nascent DNAs (Okazaki fragments) of mammalian cells1–3 and papovaviruses4,5 possess an 8–11-nucleotide RNA sequence covalently linked to their 5′ end, suggesting that this RNA sequence acts in priming in DNA synthesis. Although many studies have indicated that DNA polymerase α probably participates in the synthesis of Okazaki fragments6–10, to date, the reconstitution of de novo DNA synthesis by coupled reaction of DNA polymerase and classical RNA polymerases of mammalian cells has been unsuccessful. Here we report that a novel mouse DNA polymerase associated with primase activity11,12 synthesized DNA of ∼600 nucleotides after synthesis of initiator RNA (iRNA) of 8–10 nucleotides in the presence of a specific stimulating factor. The similarity of this DNA synthesis to that in vivo suggests the involvement of this novel DNA polymerase in the reaction required to initiate synthesis of Okazaki fragments in chromosomal DNA replication.
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Kozu, T., Yagura, T. & Seno, T. De novo DNA synthesis by a novel mouse DNA polymerase associated with primase activity. Nature 298, 180–182 (1982). https://doi.org/10.1038/298180a0
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DOI: https://doi.org/10.1038/298180a0
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