Abstract
Ca2+ is prominant in the control of cell proliferation and function1,2. However, the biochemical mechanism(s) mediating its effects on nuclear events is unknown. We report here that Ca2+, at physiological concentrations, stimulates the phosphorylation of histone H3 by an endogenous protein kinase in HeLa cell nuclei. Also, pretreatment of cells with Na butyrate, which increases histone acetylation, selectively increases the susceptibility of histone H3 to phosphorylation by the protein kinase. Our results reveal a potential link between histone H3 acetylation and phosphorylation, modifications which are thought to have important effects on chromatin structure and function and suggest a possible mechanism whereby stimuli at the cell surface (such as hormones, mitogens and drugs) may influence biochemical events at the nuclear level; changes in the intracellular Ca2+ concentration may influence the phosphorylation of chromosomal proteins, mediated by Ca2+-dependent kinases in the nucleus.
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References
Berridge, M. J. in Advances in Cyclic Nucleotide Research Vol. 11 (eds Greengard, P. & Robison, G. A.) 1–98 (Raven, New York, 1975).
Rasmussen, H. & Goodman, D. B. P. Physiol. Rev. 57, 421–509 (1977).
Simpson, R. T. Nucleic Acids Res. 5, 1109–1119 (1978).
Hnilica, L. S. The Structure and Biological Function of Histones (Chemical Rubber Co., Cleveland, 1972).
Schulman, H. & Greengard, P. Proc. natn. Acad. Sci. U.S.A. 75, 5432–5436 (1978).
Dixon, G. H. et al. Ciba Fdn Symp. 28 (new series), 229–250 (1975).
Riggs, M. G., Whittaker, R. G., Newmann, J. R. & Ingram, V. M. Nature 268, 462–464 (1977).
Boffa, L. C., Vidali, G., Mann, R. S. & Allfrey, V. G. J. biol. Chem. 253, 3364–3366 (1978).
Vidali, G., Boffa, L. C., Bradbury, E. M. & Allfrey, V. G. Proc. natn. Acad. Sci. U.S.A. 75, 2239–2243 (1978).
Candido, E. P. M., Reeves, R. & Davie, J. R. Cell 14, 105–113 (1978).
Sealy, L. & Chalkley, R. Cell 14, 115–121 (1978).
Cousens, L. S., Gallwitz, D. & Alberta, B. M. J. biol. Chem. 254, 1716–1723 (1979).
Panyim, S. & Chalkley, R. Archs Biochem. Biophys. 130, 337–346 (1969).
Elgin, S. C. R. & Weintraub, H. A. Rev. Biochem. 44, 725–774 (1975).
Kornberg, R. D. A. Rev. Biochem. 46, 931–954 (1977).
Felsenfeld, G. Nature 271, 115–122 (1978).
Isenberg, I. A. Rev. Biochem. 48, 159–191 (1979).
Gurley, L. R., D'Anna, J. A., Barkham, S. S., Deaven, L. L. & Tobey, R. A. Eur. J. Biochem. 84, 1–15 (1978).
Langan, T. A. Meth. Cell Biol. 19, 127–142 (1978).
Schulman, H. in Handbook of Experimental Pharmacology (eds Nathanson, J. A. & Kebabian, J. W.) (Springer. Berlin, in the press).
Whitlock, J. P. Jr & Simpson, R. T. Biochemistry 15, 3307–3314 (1976).
Portzehl, H., Caldwell, P. C. & Reugg, J. C. Biochim. biophys. Acta 79, 581–591 (1964).
Whitlock, J. P. Jr J. biol. Chem. 254, 5684–5689 (1979).
Simpson, R. T. & Whitlock, J. P. Jr Cell 9, 347–353 (1976).
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Whitlock, J., Augustine, R. & Schulman, H. Calcium-dependent phosphorylation of histone H3 in butyrate-treated HeLa cells. Nature 287, 74–76 (1980). https://doi.org/10.1038/287074a0
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DOI: https://doi.org/10.1038/287074a0
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