Letter | Published:

Covalent linking of photoreactive insulin to adipocytes produces a prolonged signal

Nature volume 286, pages 821822 (21 August 1980) | Download Citation

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Abstract

The first step of insulin's many cellular functions is specific binding to receptors on the plasma membrane of target cells. The subsequent molecular basis of insulin action, particularly the coupling mechanism(s) involved in transmitting the biological message, remains largely unknown1. Our approach to the problem centres on the application of a series of well characterized photo-insulins carrying an aryl–azido or nitro–aryl–azido group in positions A1, B1, B2 or B29 (refs 2–4). Specific binding to membrane components could be demonstrated with B1-, B29- (ref. 5) and A1-photo-insulins6 as well as a B2-derivative7. We now report that lipogenesis is increased to, and maintained at, near-maximal levels for several hours after photoinduced covalent binding of B2- (2-nitro,4-azidophenyl-acetyl)-des-PheB1-insulin (Napa-DP-insulin3) to living adipocytes.

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References

  1. 1.

    A. Rev. Biochem. 46, 359–384 (1977).

  2. 2.

    & in Peptides 1978–Proc. 15th Eur. Peptide Symp., Poland (eds Siemion, Z. & Kupryszewski, G.) 639–642 (Wroclaw University Press, Poland, 1979).

  3. 3.

    , & in Proc. 2nd int. Insulin Symp., Aachen (eds Brandenburg, D. & Wollmer, A.) 309–316 (De Gruyter, Berlin, 1980).

  4. 4.

    , & in Proc. 2nd int. Insulin Symp., Aachen (eds Brandenburg, D. & Wollmer, A.) 317–325 (De Gruyter, Berlin, 1980).

  5. 5.

    et al. Diabetologia 15, 282 (1978).

  6. 6.

    et al. Biochem. biophys. Res. Commun. 92, 492–498 (1980).

  7. 7.

    , , & FEBS Lett. 113, 189–192 (1980).

  8. 8.

    , , & Horm. Metab. Res. 6, 12–16 (1974).

  9. 9.

    & Biochim. biophys. Acta 320, 16–32 (1973).

  10. 10.

    , & J. biol. Chem. 250, 3368–3374 (1975).

  11. 11.

    , & , Biochemistry 19, 70–76 (1980).

  12. 12.

    et al. Nature 259, 369–373 (1976).

  13. 13.

    J. biol. Chem. 239, 375–380 (1964).

  14. 14.

    & J. Physiol., Lond. 236, 499–516 (1974).

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Author information

Affiliations

  1. Deutsches Wollforschungsinstitut, Veltmanplatz 8, D-5100 Aachen, FRG

    • Dietrich Brandenburg
    • , Cornelia Diaconescu
    • , Derek Saunders
    •  & Peter Thamm

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DOI

https://doi.org/10.1038/286821a0

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