Abstract
SOMATIC FUSION has recently been used to isolate hybrids from auxotrophic mutants of fungi1,2, yeast3 and mosses4,5. These fusions were performed by using polyethylene glycol (PEG) and/or CaCl2 solutions on protoplasts which were prepared from cells of tissues treated with enzyme mixtures to dissolve the cell wall. In the unicellular green alga Chlamydomonas mutant strains unable to make a cell wall have been isolated6,7. Such mutants, similar to plant protoplasts prepared by enzymatic digestion, can be easily grown in defined liquid or agar media8. However, they differ from true protoplasts in that they do not burst in hypotonic medium or even in distilled water, indicating that some mechanism of osmoregulation is present6. It was thus of interest to determine whether these cell wall mutants could be used as plant protoplasts in fusion experiments. We report here the isolation of stable fusion products obtained from cell wall haploid mutants of Chlamydomonas reinhardi having the same mating type (mt+) and bearing genetic markers of auxotrophy.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ferenczy, L., Kevei, F. & Zsolt, J. Nature 248, 793–794 (1974).
Anné, J. & Peberdy, J. F. Archs Microbiol. 105, 201–205 (1975).
Sipiczki, M. & Ferenczy, L. Molec. gen. Genet. 151, 77–81 (1977).
Grimsley, N. H., Ashton, N. W. & Cove, D. J. Molec. gen. Genet. 154, 97–100 (1977).
Schieder, O. Z. Pflanzenphysiol. 74, 357–365 (1974).
Davies, D. R. & Plaskitt, A. Genet. Res. 17, 33–43 (1971).
Loppes, R. & Deltour, R. Archs Microbiol. 103, 247–250 (1975).
Gresshoff, P. M. Aust. J. Pl. Physiol. 3, 457–464 (1976).
Matagne, R. F. Molec. gen. Genet. 160, 95–99 (1978).
Loppes, R., Matagne, R. F. & Strijkert, P. J. Heredity 28, 239–251 (1972).
Loppes, R. Z. Vererbungslehre 98, 193–202 (1966).
Giles, K. W. & Myers, A. Nature 206, 93 (1965).
Ledoux, L., Huart, R. & Jacobs, M. Eur. J. Biochem. 23, 96–108 (1971).
Lurquin, P., Tshitenge, G., Delaunoit, G. & Ledoux, L. Analyt. Biochem. 65, 1–10 (1975).
Chiang, E. S. Aust. Acad. sci. Symp. (eds Boardman, N. K., Linnane, A. W. & Smillie, R. M.) 235 (North-Holland, Amsterdam, 1971).
Lane, B. D. & Sager, R. Cytoplasmic genes and organelles (ed. Sager, R.) 405 (Academic, New York, 1972).
Levine, R. P. & Ebersold, N. T. Cold Spring Harb. Symp. quant. Biol. 23, 101–105 (1958).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MATAGNE, R., DELTOUR, R. & LEDOUX, L. Somatic fusion between cell wall mutants of Chlamydomonas reinhardi. Nature 278, 344–346 (1979). https://doi.org/10.1038/278344a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/278344a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.